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van Zyl, W.H. et al.

van Zyl, Eliasson, Hobley, Hahn-Hägerdal,

Xylose utilisation by recombinant strains of Saccharomyces cerevisiae on different carbon sources.

Autoselective xylose-utilising strains of Saccharomyces cerevisiae expressing the xylose reductase (XYL1) and xylitol dehydrogenase (XYL2) genes of Pichia stipitis were constructed by replacing the chromosomal FUR1 gene with a disrupted fur1::LEU2 allele. Anaerobic fermentations with 80 g l-1 D-xylose as substrate showed a twofold higher consumption of xylose in complex medium compared to defined medium. The xylose consumption rate increased a further threefold when 20 g l-1 D-glucose or raffinose was used as co-substrate together with 50 g l-1 D-xylose. Xylose consumption was higher with raffinose as co-substrate than with glucose (85% versus 71%, respectively) after 82 h fermentations. A high initial ethanol concentration and moderate levels of glycerol and acetic acid accompanied glucose as co-substrate, whereas the ethanol concentration gradually increased with raffinose as co-substrate with no glycerol and much less acetic acid formation.[1]

References

Xylose utilisation by recombinant strains of Saccharomyces cerevisiae on different carbon sources. van Zyl, W.H., Eliasson, A., Hobley, T., Hahn-Hägerdal, B. Appl. Microbiol. Biotechnol. (1999) [Pubmed]

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