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Lustig, L.R. et al.

Lustig, Peng, Hiel, Yamamoto, Fuchs,

Molecular cloning and mapping of the human nicotinic acetylcholine receptor alpha10 (CHRNA10).

We report the isolation and initial characterization of a new member of the human nicotinic acetylcholine receptor (nAChR) subunit family, alpha10 (CHRNA10), from both inner-ear neuroepithelium and lymphoid tissue. The cDNA is 1959 nucleotides in length, with a coding region predicting a protein of 451 amino acids that is 90% identical to rat alpha10. The alpha10 gene was localized to chromosome 11p15. 5. Human alpha10 was detected in human inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes using reverse transcriptase-polymerase chain reaction, Northern blot hybridization, and immunohistochemistry. We also detected the expression of the human nAChR alpha9 (CHRNA9) mRNA in these same tissues using RT-PCR and Northern blot hybridization.[1]

References

Molecular cloning and mapping of the human nicotinic acetylcholine receptor alpha10 (CHRNA10). Lustig, L.R., Peng, H., Hiel, H., Yamamoto, T., Fuchs, P.A. Genomics (2001) [Pubmed]

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