The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Laxifolone A suppresses LPS/IFN-gamma- induced NO synthesis by attenuating NF-kappaB translocation: role of NF-kappaB p105 level.

Laxifolone A is a triterpene isolated from Euonymus laxiflorus Champ. Exposure of RAW264.7 macrophages to laxifolone A concentration-dependently suppressed lipopolysaccharide/interferon-gamma (LPS/IFN-gamma)-induced nitrite production (IC50 = 0.37 +/- 0.05 microM), inducible NO synthase (iNOS) protein, and iNOS mRNA expression. Translocation of nuclear factor-kappaB (NF-kappaB) into the nucleus with subsequent activation of iNOS gene transcription is essential in NO signaling. Western blot analysis indicated that the cytosolic NF-kappaB/ p65 was obviously decreased after LPS/IFN-gamma stimulation for 30 min and this phenomenon could be reversed by laxifolone A. Similarly, a time-related NF-kappaB/ p65 nuclear translocation induced by LPS/IFN-gamma was diminished in the presence of laxifolone A. However, laxifolone A failed to interfere in LPS/IFN-gamma-evoked IkappaB degradation. Our results also showed that LPS/IFN-gamma-stimulation resulted in the degradation of NF-kappaB p105, the NF-kappaB precursor, and laxifolone A treatment significantly counteracted this effect. Furthermore, laxifolone A itself was able to enhance NF-kappaB p105 protein expression. In summary, these results suggest that inhibition of NF-kappaB p105 degradation in cytoplasm may participate in the abrogation of LPS/IFN-gamma- induced NF-kappaB translocation and subsequent NO synthesis by laxifolone A.[1]

References

 
WikiGenes - Universities