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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Immunohistochemical location of placental proteins (PP8, 9, 10, 11, 12) in human term placentae.

By using an immunoglobulin enzyme bridge ( PAP) technique, the location of two ubiquitous tissue proteins (PP8, PP9) and three placental specific tissue proteins (PP10, PP11, PP12) was investigated in ethanol/acetic acid-fixed paraffin-embedded human term placentae. PP8 was found mainly in both the cytoplasm and the nucleus of trophoblast cells (chorion) and syncytiotrophoblast (villi). PP9 was found in the cytoplasm of trophoblast cells (chorion), the fibrous part of interstitial connective tissues (villi), and the cytoplasm of histiocytes (villi, amnion, and decidua). PP10 was found in the cytoplasm of epithelium (amnion) and in both the cytoplasm and the nucleus of syncytiotrophoblast (villi). PP12 was found in the cytoplasm of histiocytes in decidua, amnion, and chorion and the cytoplasm of syncytiotrophoblast (villi). In addition, stains for PP8, PP10, and PP12 were taken up by the cytoplasm of histiocytes found in amnion, villi, the intervillous space, and decidua, in contrast to PP11, which was found to be specific to the cytoplasm of trophoblast cells (chorion and villi). Possible clinical applications of these findings are discussed.[1]

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