Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Satoh, Y. et al.

Carbamylcholine- and catecholamine-induced intracellular calcium dynamics of epithelial cells in mouse ileal crypts.

BACKGROUND/AIMS: The intestinal epithelium is composed of various cells, and the enteric nervous system, which controls the epithelial functions, has different neurotransmitters and/or modulators. The aim of this study was to show whether the responses of intestinal epithelial cells to different neurotransmitters are elicited throughout the entire epithelium or are restricted to a certain cell. METHODS: The spatiotemporal dynamics of cytosolic calcium ion ([Ca2+]c) were measured by digital imaging analysis in isolated crypts of mouse ileum loaded with [1-[2-(5'-carboxyoxazol-2'-yl)-6-amino-benzofuran-5- oxy]-2-(2'-amino-5'-methylphenoxy)-ethane-N,N,N',N'-tetraacetic acid] pentakis (acetoxylmethyl) ester. Thereafter, the crypt cells were identified morphologically. RESULTS: Carbamylcholine elicited [Ca2+]c dynamics in Paneth cells, showing a biphasic increase, but neither cholecystokinin octapeptide nor nicotine had any effect on the [Ca2+]c of the crypt cells including the Paneth cells. Adrenaline and noradrenaline, but not isoproterenol, induced a transient elevation of [Ca2+]c of some enterochromaffin cells. Increases in the [Ca2+]c of most crypt cells were elicited by thapsigargin. Propagation of a [Ca2+]c wave in the crypts was not evident. CONCLUSIONS: Increases in [Ca2+]c can be induced by carbamylcholine in Paneth cells and catecholamines in some enterochromaffin cells. The digital imaging analysis showed the heterogeneity of the responses of intestinal crypt cells to different transmitters.[1]

References

Carbamylcholine- and catecholamine-induced intracellular calcium dynamics of epithelial cells in mouse ileal crypts. Satoh, Y., Habara, Y., Ono, K., Kanno, T. Gastroenterology (1995) [Pubmed]

Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.