Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Chemical Compound Review:

L-Uridine 1-[(2S,3S,4R,5S)-3,4- dihydroxy-5...

Synonyms: AC1LADJT, SureCN330119, AG-E-82390, CTK8G0686, ZINC00895165, ...

Temmink, O.H. et al., Falcone, A. et al., Laurensse, E.J. et al., Calabresi, P. et al., Rima, B.K. et al., et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of uridine

However, the range of effective doses is narrow, and higher doses of Urd (greater than 3,000 mg/kg/d) significantly enhance hematologic toxicity [1].
Increased extracellular concentrations of uridine (Urd) have been reported to reduce, in vitro, azidothymidine (AZT)-induced inhibition of human granulocyte-macrophage progenitor cells without impairment of its antihuman immunodeficiency virus (HIV) activity [2].
In the colon cancer cells 5'DFUR degradation varied from 0.4 to 50nmol5FU/h/10(6)cells, that of TdR from 0.3 to 103nmolthymine/h/10(6)cells, that of Urd from 0.8 to 79nmoluracil/h/10(6)cells, while conversion of 5FU to FUrd was from 0.3 to 46nmol/h/10(6)cells [3].
In Bacillus subtilis, uracil (Ura), uridine (Urd), and deoxyuridine (dUrd) are metabolized through pathways similar to those of enteric bacteria [4].

High impact information on uridine

Our studies in mice have shown that daily oral doses of benzylacyclouridine (BAU), an inhibitor of Urd breakdown, also reduces AZT hematologic toxicity, presumably by elevating the plasma concentration of Urd [1].
Because the relative contribution of each of the two enzymes to these reactions is still largely unknown, we investigated the substrate specificity of TP and UP in colon cancer cells for the (fluoro)pyrimidine nucleosides thymidine (TdR), uridine (Urd), 5'-deoxy-5-fluorouridine (5'DFUR), and 5FU [3].
The main enzyme of PNP is Urd phosphorylase in rodents and dThd phosphorylase in humans [5].
The assay has been used to measure kinetic parameters for 5'dFUR and UR in two cell lines from intestinal origin [6].

Biological context of uridine

In conclusion, overlapping substrate specificity was found for TP and UP in the cell lines, in which both enzymes were responsible for converting TdR and Urd, and 5'DFUR [3].

Anatomical context of uridine

In mice receiving concomitant AZT, daily doses of Urd of 1,000 to 2,000 mg/kg increase peripheral reticulocytes and slightly reduce AZT-induced hematologic toxicity [1].

Associations of uridine with other chemical compounds

With the natural substrate uridine (UR) and the analog, 5'-deoxy-5-fluorouridine (5'dFUR) conditions have been optimized to measure the product formation with reversed-phase high-performance liquid chromatography [6].

References

Different effect of benzylacyclouridine on the toxic and therapeutic effects of azidothymidine in mice. Falcone, A., Darnowski, J.W., Ruprecht, R.M., Chu, S.H., Brunetti, I., Calabresi, P. Blood (1990) [Pubmed]
Benzylacyclouridine reverses azidothymidine-induced marrow suppression without impairment of anti-human immunodeficiency virus activity. Calabresi, P., Falcone, A., St Clair, M.H., Wiemann, M.C., Chu, S.H., Darnowski, J.W. Blood (1990) [Pubmed]
Activity and substrate specificity of pyrimidine phosphorylases and their role in fluoropyrimidine sensitivity in colon cancer cell lines. Temmink, O.H., de Bruin, M., Turksma, A.W., Cricca, S., Laan, A.C., Peters, G.J. Int. J. Biochem. Cell Biol. (2007) [Pubmed]
Metabolism of pyrimidine bases and nucleosides in Bacillus subtilis. Rima, B.K., Takahashi, I. J. Bacteriol. (1977) [Pubmed]
Species differences in substrate specificity of pyrimidine nucleoside phosphorylase. Maehara, Y., Sakaguchi, Y., Kusumoto, T., Kusumoto, H., Sugimachi, K. Journal of surgical oncology. (1989) [Pubmed]
A sensitive non-radioactive assay for pyrimidine nucleoside phosphorylase using reversed-phase high performance liquid chromatography. Laurensse, E.J., Pinedo, H.M., Peters, G.J. Clin. Chim. Acta (1988) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.