The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Chemical Compound Review

deoxyinosine     9-[(2R,4S,5R)-4-hydroxy-5...

Synonyms: delta-Ino, d-Ino, SureCN24307, SureCN24308, AG-H-60709, ...
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of deoxyinosine

 

High impact information on deoxyinosine

  • The differences between the normal and HPR transferase-deficient cells were lost when ribose reutilization from endogenous nucleotide breakdown was impaired in the HPR transferase-deficient cells by incubation with 2'-deoxyinosine [3].
  • In this study, we demonstrated the artifactual formation of these two deamination products during acid hydrolysis and hence developed a method for detecting and measuring 2'-deoxyinosine, the nucleoside of hypoxanthine [4].
  • This assay detected an increase in the levels of 2'-deoxyinosine in DNA when commercial salmon testis DNA was treated with nitrous acid [4].
  • Instead, the results show a very small shift (1.3 ppm), indicating that C-6 of 2'-deoxyinosine retains its sp2 hybridization after binding by calf intestinal adenosine deaminase [5].
  • We show that dI-substituted molecules that cannot form the G-strand structure nonetheless function as substrates for telomere repeat addition in vitro by the telomere lengthening enzyme, telomerase [6].
 

Biological context of deoxyinosine

  • PURPOSE: The aims of this study were to develop a stealth, pegylated liposomal formulation of 2'-deoxyinosine (d-Ino), a 5-fluorouracil (5-FU) modulator, to evaluate its efficacy in vitro and in tumor-bearing mice, and to study its pharmacokinetics in rats [7].
  • The sensitivity was also increased by a factor of 10 by using in addition to the deoxyinosine (dI)-containing primers, respective homologous primers in which dI was substituted by dG in the region of sequence homology [8].
  • The inhibitory potency of this siHybrid was increased (IC50 value of 7.8 nM) using a homologous chemically modified siHybrid (mH) in which the 19-mer sense strand had the following pattern of 2 '-deoxyinosine (dI) and 2 '-O-methylribonucleotide (2 '-OMe) residues: 5'-d(TITIT)-2'OMe(GCGGUGGUU)-d(GICIT) [9].
 

Associations of deoxyinosine with other chemical compounds

 

Gene context of deoxyinosine

 

Analytical, diagnostic and therapeutic context of deoxyinosine

  • Our assay for 2'-deoxyinosine employs nuclease P1 and alkaline phosphatase to achieve release of the nucleosides from DNA, followed by HPLC prepurification with subsequent GC-MS analysis of the nucleosides [4].

References

  1. Incorporation of oxidatively modified 2'-deoxynucleotide triphosphates by HIV-1 RT on RNA and DNA templates. Wuenschell, G.E., Valentine, M.R., Termini, J. Chem. Res. Toxicol. (2002) [Pubmed]
  2. One-pot Microbial Synthesis of 2'-deoxyribonucleoside from Glucose, Acetaldehyde, and a Nucleobase. Horinouchi, N., Ogawa, J., Kawano, T., Sakai, T., Saito, K., Matsumoto, S., Sasaki, M., Mikami, Y., Shimizu, S. Biotechnol. Lett. (2006) [Pubmed]
  3. The influence of ribose 5-phosphate availability on purine synthesis of cultured human lymphoblasts and mitogen-stimulated lymphocytes. Pilz, R.B., Willis, R.C., Boss, G.R. J. Biol. Chem. (1984) [Pubmed]
  4. Potential artifacts in the measurement of DNA deamination. Lim, K.S., Huang, S.H., Jenner, A., Wang, H., Tang, S.Y., Halliwell, B. Free Radic. Biol. Med. (2006) [Pubmed]
  5. Enzyme-substrate complexes of adenosine and cytidine deaminases: absence of accumulation of water adducts. Shih, P., Wolfenden, R. Biochemistry (1996) [Pubmed]
  6. Telomere G-strand structure and function analyzed by chemical protection, base analogue substitution, and utilization by telomerase in vitro. Henderson, E.R., Moore, M., Malcolm, B.A. Biochemistry (1990) [Pubmed]
  7. Development of stealth liposome formulation of 2'-deoxyinosine as 5-fluorouracil modulator: in vitro and in vivo study. Fanciullino, R., Giacometti, S., Aubert, C., Fina, F., Martin, P.M., Piccerelle, P., Ciccolini, J. Pharm. Res. (2005) [Pubmed]
  8. A spot multiplex nested RT-PCR for the simultaneous and generic detection of viruses involved in the aetiology of grapevine leafroll and rugose wood of grapevine. Dovas, C.I., Katis, N.I. J. Virol. Methods (2003) [Pubmed]
  9. Chemically modified short interfering hybrids (siHYBRIDS): nanoimmunoliposome delivery in vitro and in vivo for RNAi of HER-2. Hogrefe, R.I., Lebedev, A.V., Zon, G., Pirollo, K.F., Rait, A., Zhou, Q., Yu, W., Chang, E.H. Nucleosides Nucleotides Nucleic Acids (2006) [Pubmed]
  10. Inosine and 2'-deoxyinosine and their synthetic analogues: lipophilicity in the relation to their retention in reversed-phase liquid chromatography and the stability characteristics. Novotny, L., Abdel-Hamid, M., Hamza, H. Journal of pharmaceutical and biomedical analysis. (2000) [Pubmed]
  11. Reduced activity of anabolizing enzymes in 5-fluorouracil-resistant human stomach cancer cells. Inaba, M., Mitsuhashi, J., Sawada, H., Miike, N., Naoe, Y., Daimon, A., Koizumi, K., Tsujimoto, H., Fukushima, M. Jpn. J. Cancer Res. (1996) [Pubmed]
  12. Early postimplantation embryolethality in mice following in utero inhibition of adenosine deaminase with 2'-deoxycoformycin. Knudsen, T.B., Gray, M.K., Church, J.K., Blackburn, M.R., Airhart, M.J., Kellems, R.E., Skalko, R.G. Teratology (1989) [Pubmed]
 
WikiGenes - Universities