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Gene Review:

uidA - beta-D-glucuronidase

Escherichia coli CFT073

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Disease relevance of uidA

Expression of pylTSBCD also suppressed an amber codon introduced into the E. coli uidA gene [1].
Beta-glucuronidase-negative, sorbitol-nonfermenting isolates of Shiga toxin-producing Escherichia coli O157 comprise part of a clone complex of related enterohemorrhagic E. coli isolates [2].
HIV protease-activated molecular switches based on beta-glucuronidase and alkaline phosphatase [3].
The manA gene along with a beta-glucuronidase (GUS) gene was transferred into apple cv. 'Holsteiner Cox' via Agrobacterium tumefaciens-mediated transformation [4].

High impact information on uidA

Here we report that atypical, beta-glucuronidase-positive and sorbitol-fermenting, strains of EHEC O157 harbor intact tccP and tccP2 genes, both of which are secreted by the LEE-encoded type III secretion system [5].
Cysteine-quenched derivatives of 9b and 17 were determined to be substrates for E. coli beta-glucuronidase, resulting in facile drug release [6].
Random Elongation Mutagenesis [1] was used to fuse the p6 peptide, which is recognized and cleaved by HIV protease, and twelve random sequence amino acids to the C-termini of beta-glucuronidase (GUS) and alkaline phosphatase (AP) [3].
Acidic and enzymatic hydrolysis with beta-glucuronidase from Escherichia coli and Helix pomatia were evaluated [7].
We transformed the N. lolii strain Lp19 with plasmids, in which DNA fragments upstream of the open reading frame of the N. lolii HMG CoA reductase gene controlled expression of the GUS (gusA; Escherichia coli beta-glucuronidase) reporter gene [8].

Chemical compound and disease context of uidA

Aims: Enzyme substrates based on 4-methylumbelliferone are widely used for the detection of Escherichia coli and enterococci in water, by detection of beta-glucuronidase and beta-glucosidase activity respectively [9].
E. coli beta-glucuronidase hydrolysis MDA recovery was 17.1% and 26.5% greater than acid hydrolysis and H. pomatia beta-glucuronidase recovery (SE = 3.3 and 6.1%), respectively [7].

Biological context of uidA

Beta-glucuronidase as reporter gene: advantages and limitations [10].

Analytical, diagnostic and therapeutic context of uidA

After confirming the identity of 184 environmental bacterial isolates as E. coli, each was subjected to polymerase chain reaction (PCR) of the beta-glucuronidase gene (uidA) followed by DGGE fingerprinting [11].

References

A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine. Longstaff, D.G., Larue, R.C., Faust, J.E., Mahapatra, A., Zhang, L., Green-Church, K.B., Krzycki, J.A. Proc. Natl. Acad. Sci. U.S.A. (2007) [Pubmed]
Identification of Escherichia coli O157:H7 Genomic Regions Conserved in Strains with a Genotype Associated with Human Infection. Steele, M., Ziebell, K., Zhang, Y., Benson, A., Konczy, P., Johnson, R., Gannon, V. Appl. Environ. Microbiol. (2007) [Pubmed]
HIV protease-activated molecular switches based on beta-glucuronidase and alkaline phosphatase. O'Loughlin, T.L., Matsumura, I. Comb. Chem. High Throughput Screen. (2006) [Pubmed]
The use of the phosphomannose-isomerase/mannose selection system to recover transgenic apple plants. Degenhardt, J., Poppe, A., Montag, J., Szankowski, I. Plant Cell Rep. (2006) [Pubmed]
TccP2 of O157:H7 and non-O157 enterohemorrhagic Escherichia coli (EHEC): challenging the dogma of EHEC-induced actin polymerization. Ogura, Y., Ooka, T., Whale, A., Garmendia, J., Beutin, L., Tennant, S., Krause, G., Morabito, S., Chinen, I., Tobe, T., Abe, H., Tozzoli, R., Caprioli, A., Rivas, M., Robins-Browne, R., Hayashi, T., Frankel, G. Infect. Immun. (2007) [Pubmed]
Development and properties of beta-glucuronide linkers for monoclonal antibody-drug conjugates. Jeffrey, S.C., Andreyka, J.B., Bernhardt, S.X., Kissler, K.M., Kline, T., Lenox, J.S., Moser, R.F., Nguyen, M.T., Okeley, N.M., Stone, I.J., Zhang, X., Senter, P.D. Bioconjug. Chem. (2006) [Pubmed]
Selection and Optimization of Hydrolysis Conditions for the Quantification of Urinary Metabolites of MDMA. Pirnay, S.O., Abraham, T.T., Lowe, R.H., Huestis, M.A. Journal of analytical toxicology (2006) [Pubmed]
Transformation of the ryegrass endophyte Neotyphodium lolii can alter its in planta mycelial morphology. Zhang, N., Scott, V., Al-Samarrai, T.H., Tan, Y.Y., Spiering, M.J., McMillan, L.K., Lane, G.A., Scott, D.B., Christensen, M.J., Schmid, J. Mycol. Res. (2006) [Pubmed]
Evaluation of novel fluorogenic substrates for the detection of glycosidases in Escherichia coli and enterococci. Perry, J.D., James, A.L., Morris, K.A., Oliver, M., Chilvers, K.F., Reed, R.H., Gould, F.K. J. Appl. Microbiol. (2006) [Pubmed]
Beta-glucuronidase as reporter gene: advantages and limitations. Kim, K.W., Franceschi, V.R., Davin, L.B., Lewis, N.G. Methods Mol. Biol. (2006) [Pubmed]
Evaluation of denaturing gradient gel electrophoresis to differentiate Escherichia coli populations in secondary environments. Sigler, V., Pasutti, L. Environ. Microbiol. (2006) [Pubmed]

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