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Gene Review

manA  -  mannose-6-phosphate isomerase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1608, JW1605, pmi
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Disease relevance of manA


High impact information on manA

  • T1 is located near 28.5 min, which is adjacent to trp, and T2 is located at 34.5-35.7 min on the opposite side of the terminus region, near manA [6].
  • The DNA fragment also contains the 5' end of the manA gene encoding mannose 6-phosphate isomerase, which is transcribed with the opposite polarity to fumA [7].
  • Incorporation of radioactive mannose into the phosphomannose isomerase-deficient strain E. coli F860 O9 pmi allowed the derivation of the number of O-antigens per cell required to increase T5 adsorption [8].
  • Therefore, we introduced the Escherichia coli gene pmi (manA) in Z. mobilis under the control of a lacIq-Ptac system on a broad-host-range plasmid (pZY507; Cmr) [9].
  • The cloned gene was sequenced; there was 77.4% nucleotide homology between the cloned pmi gene and the analogous manA gene of E. coli K-12, and 86.2% amino acid sequence homology between their presumptive gene products [5].

Chemical compound and disease context of manA


Biological context of manA


Associations of manA with chemical compounds


Other interactions of manA

  • The defective gene in one of these mutants, dicA, was mapped at 34.9 min by linkage with manA and with three physically characterized Tn10 insertions [15].
  • However, we have recently identified this ORF as the putative pgi/pmi gene coding for hypothetical bifunctional phosphoglucose/phosphomannose isomerase (PGI/PMI) [16].

Analytical, diagnostic and therapeutic context of manA


  1. Nucleotide sequence and transcriptional start point of the phosphomannose isomerase gene (manA) of Escherichia coli. Miles, J.S., Guest, J.R. Gene (1984) [Pubmed]
  2. Nucleotide sequence analysis of the phosphomannose isomerase gene (pmi) of Pseudomonas aeruginosa and comparison with the corresponding Escherichia coli gene manA. Darzins, A., Frantz, B., Vanags, R.I., Chakrabarty, A.M. Gene (1986) [Pubmed]
  3. The use of the phosphomannose-isomerase/mannose selection system to recover transgenic apple plants. Degenhardt, J., Poppe, A., Montag, J., Szankowski, I. Plant Cell Rep. (2006) [Pubmed]
  4. Molecular characterization and utilization of the CAK1 filamentous viruslike particle derived from Clostridium beijerinckii. Li, Y., Blaschek, H.P., Tan, D. J. Ind. Microbiol. Biotechnol. (2002) [Pubmed]
  5. Sequence of the phosphomannose isomerase-encoding gene of Salmonella typhimurium. Collins, L.V., Hackett, J. Gene (1991) [Pubmed]
  6. The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication. Hill, T.M., Henson, J.M., Kuempel, P.L. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  7. Complete nucleotide sequence of the fumarase gene fumA, of Escherichia coli. Miles, J.S., Guest, J.R. Nucleic Acids Res. (1984) [Pubmed]
  8. Polymannose O-antigens of Escherichia coli, the binding sites for the reversible adsorption of bacteriophage T5+ via the L-shaped tail fibers. Heller, K., Braun, V. J. Virol. (1982) [Pubmed]
  9. Expression of the Escherichia coli pmi gene, encoding phosphomannose-isomerase in Zymomonas mobilis, leads to utilization of mannose as a novel growth substrate, which can be used as a selective marker. Weisser, P., Krämer, R., Sprenger, G.A. Appl. Environ. Microbiol. (1996) [Pubmed]
  10. Alginate biosynthetic enzymes in mucoid and nonmucoid Pseudomonas aeruginosa: overproduction of phosphomannose isomerase, phosphomannomutase, and GDP-mannose pyrophosphorylase by overexpression of the phosphomannose isomerase (pmi) gene. Sá-Correia, I., Darzins, A., Wang, S.K., Berry, A., Chakrabarty, A.M. J. Bacteriol. (1987) [Pubmed]
  11. A mannanase, ManA, of the polycentric anaerobic fungus Orpinomyces sp. strain PC-2 has carbohydrate binding and docking modules. Ximenes, E.A., Chen, H., Kataeva, I.A., Cotta, M.A., Felix, C.R., Ljungdahl, L.G., Li, X.L. Can. J. Microbiol. (2005) [Pubmed]
  12. Agrobacterium tumefaciens-mediated sorghum transformation using a mannose selection system. Gao, Z., Xie, X., Ling, Y., Muthukrishnan, S., Liang, G.H. Plant Biotechnol. J. (2005) [Pubmed]
  13. Cloning of Escherichia coli and Pseudomonas aeruginosa phosphomannose isomerase genes and their expression in alginate-negative mutants of Pseudomonas aeruginosa. Darzins, A., Nixon, L.L., Vanags, R.I., Chakrabarty, A.M. J. Bacteriol. (1985) [Pubmed]
  14. Agrobacterium and biolistic transformation of onion using non-antibiotic selection marker phosphomannose isomerase. Aswath, C.R., Mo, S.Y., Kim, D.H., Park, S.W. Plant Cell Rep. (2006) [Pubmed]
  15. A new dispensable genetic locus of the terminus region involved in control of cell division in Escherichia coli. Béjar, S., Bouché, J.P. Mol. Gen. Genet. (1985) [Pubmed]
  16. Bifunctional phosphoglucose/phosphomannose isomerase from the hyperthermophilic archaeon Pyrobaculum aerophilum. Hansen, T., Urbanke, C., Schönheit, P. Extremophiles (2004) [Pubmed]
  17. In vivo performance of a dual genetic marker, manA-gfp, in transgenic bentgrass. Fu, D., Xiao, Y., Muthukrishnan, S., Liang, G.H. Genome (2005) [Pubmed]
  18. Isolation and sequence analysis of the pmi gene encoding phosphomannose isomerase of Streptococcus mutans. Sato, Y., Yamamoto, Y., Kizaki, H., Kuramitsu, H.K. FEMS Microbiol. Lett. (1993) [Pubmed]
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