Disease relevance of rpoE

• Based on the similarity between ORF1 and Escherichia coli rpoE, we have tentatively designated this locus the rpoE locus [1].
• An rpoE-like locus controls outer membrane protein synthesis and growth at cold temperatures and high pressures in the deep-sea bacterium Photobacterium sp. strain SS9 [1].
• The rpoE gene encoding an RNA polymerase sigmaE subunit was isolated from a gamma-phage library of the deep-sea piezophilic and psychrophilic bacterium Shewanella violacea strain DSS12 [2].

High impact information on rpoE

• Most of these revertants possessed DNA rearrangements at the site of the transposon insertion, further demonstrating the importance of the rpoE locus to high-pressure and cold-temperature growth [1].
• Although able to invade both macrophage and epithelial cell lines normally, the rpoE mutant was defective in its ability to survive and proliferate in both cell lines [3].
• However, the htrA mutant efficiently colonized the livers and spleens of mice infected i.v., but the rpoE mutant did not [3].
• Cell lysis directed by sigmaE in early stationary phase and effect of induction of the rpoE gene on global gene expression in Escherichia coli [4].
• Induction of the T. maritima rpoE/ sigW and rpoD/ sigA homologs suggests a mechanism for global heat-shock response in the absence of an identifiable ortholog to a major heat-shock sigma factor [5].

Biological context of rpoE

• Comparison of the promoters revealed a consensus sequence almost identical to the sigma(E)-dependent rpoEp3 promoter directing expression of rpoE [6].

Analytical, diagnostic and therapeutic context of rpoE

• Further analysis of the lysis phenomenon was performed using a transient expression system of the rpoE gene and by DNA microarray [4].
• The microarray analysis with RNAs from exponential and early stationary phase cells revealed that a large number of genes were up- or down-regulated when the rpoE gene was induced, and that several genes were induced in a phase-specific manner [4].

References