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Gene Review:

Tg(Tek-cre)12Flv - transgene insertion 12, Richard A Flavell

Mus musculus

This record was discontinued.

Li, W.L. et al., Kisanuki, Y.Y. et al., Gitler, A.D. et al., Shimoda, M. et al., Braren, R. et al.

Li, Cheng, Tan, Zhang, Sun, Chen, Yang,

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Disease relevance of Tg(Tek-cre)12Flv

Tie2-Cre-induced inactivation of a conditional mutant Nf1 allele in mouse results in a myeloproliferative disorder that models juvenile myelomonocytic leukemia [1].

High impact information on Tg(Tek-cre)12Flv

To investigate the cellular mechanism underlying this process, we deleted focal adhesion kinase (FAK), a key signaling mediator, in endothelial cells (ECs) using Tie2-Cre mice [2].
The latter was accomplished by conditional deletion of a loxP-flanked MHC-II beta-chain allele using a TIE2Cre transgene, which causes a very high degree of deletion in hemopoietic/endothelial progenitor cells but without deletion among thymic epithelial cells [3].
Tie2-Cre transgenic mice: a new model for endothelial cell-lineage analysis in vivo [4].
Here, we created Tie2-Cre transgenic mice, in which expression of Cre recombinase is driven by an endothelial-specific promoter/enhancer [4].
Tie2-Cre transgenic mice express Cre recombinase in all endothelial cells [1].

Biological context of Tg(Tek-cre)12Flv

Total six founder mice carrying the Tie2-Cre transgene were identified by genomic PCR and Southern blot [5].
In order to test the excision activity and tissue distribution of the Cre recombinase, the Tie2-Cre transgenic line was crossed with the mouse strain carrying the Smad4 conditional alleles (Smad4(Co/Co)) or the reporter line ROSA26 [5].

Other interactions of Tg(Tek-cre)12Flv

Here, we show that Tie2-Cre-mediated deletion of Nf1 also leads to excision of Nf1 in the hematopoietic lineage [1].

Analytical, diagnostic and therapeutic context of Tg(Tek-cre)12Flv

PCR of multiple tissue DNA from Tie2-Cre; Smad4(Co/+) mice revealed the Cre activity in all tissues containing endothelial cells [5].
Tie2-Cre transgenic mice are a new genetic tool for the analyses of endothelial cell-lineage and endothelial cell-specific gene targeting [4].

References

Tie2-Cre-induced inactivation of a conditional mutant Nf1 allele in mouse results in a myeloproliferative disorder that models juvenile myelomonocytic leukemia. Gitler, A.D., Kong, Y., Choi, J.K., Zhu, Y., Pear, W.S., Epstein, J.A. Pediatr. Res. (2004) [Pubmed]
Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation. Braren, R., Hu, H., Kim, Y.H., Beggs, H.E., Reichardt, L.F., Wang, R. J. Cell Biol. (2006) [Pubmed]
Conditional ablation of MHC-II suggests an indirect role for MHC-II in regulatory CD4 T cell maintenance. Shimoda, M., Mmanywa, F., Joshi, S.K., Li, T., Miyake, K., Pihkala, J., Abbas, J.A., Koni, P.A. J. Immunol. (2006) [Pubmed]
Tie2-Cre transgenic mice: a new model for endothelial cell-lineage analysis in vivo. Kisanuki, Y.Y., Hammer, R.E., Miyazaki , J., Williams, S.C., Richardson, J.A., Yanagisawa, M. Dev. Biol. (2001) [Pubmed]
Endothelial cell-specific expression of Cre recombinase in transgenic mice. Li, W.L., Cheng, X., Tan, X.H., Zhang, J.S., Sun, Y.S., Chen, L., Yang, X. Yi Chuan Xue Bao (2005) [Pubmed]

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