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Gene Review

Emv3  -  endogenous ecotropic MuLV 3

Mus musculus

Synonyms: Emv-3, Sev-1
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Disease relevance of Emv3

  • DBA/2 mice carry a single endogenous ecotropic murine leukemia provirus, Emv-3 [1].
  • In addition, most of the replication-competent viruses that were sequenced appeared to result from simple mutation of Emv-3 rather than recombination with other endogenous murine leukemia viruses [1].
  • We characterized an infectious ecotropic retrovirus spontaneously expressed in aged DBA/2 mice, which carries the complete but defective Emv-3 [2].
  • The results of this study conclusively demonstrated that Emv-3 is not causally associated with the development of DMBA-induced lymphomas in DBA/2J mice [3].
  • A replication-competent, endogenous retrovirus from an aged DBA/2 mouse contains the complete env from Emv-3 and a novel gag partially related to AKT-8 [4].

High impact information on Emv3

  • We report here the entire sequence for the gag gene of rv-DBA/2aged as well as the previously unsequenced 3' end of the Emv-3 gag gene [4].
  • The previous report showed that a recombination which resulted in the replacement of Emv-3 gag sequences with gag sequences homologous to those found in the AKT-8 virus had taken place [4].
  • To test this possibility, we compared lymphoma incidence after percutaneous DMBA treatment in DBA/2J-dv/dv mice, which carry two copies of Emv-3, with lymphoma incidence in DBA/2J-d+18J/d+18J mice, which lost both copies of Emv-3 by homologous recombination involving the long terminal repeat sequences [3].
  • Comparison of the nucleotide sequence of the Emv-3 provirus with the sequence of the highly infectious Akv murine leukemia virus revealed three nucleotide differences within the gag coding region [5].
  • Thus, it seems most likely that the defect of expression of the Emv-3 provirus is due to the presence of a proline is position 3 of the gag polyprotein, preventing the myristylation [5].

Biological context of Emv3


Associations of Emv3 with chemical compounds


Regulatory relationships of Emv3


Other interactions of Emv3

  • Marker rescue experiments suggested that Emv-3 is defective in the gag region and Emv-13 is defective in p15E-U3 [7].
  • Some recombinant ecotropic viruses contained sequences in the p15E-U3 region that were not derived from Emv-1 and Emv-3 but were found in recombinant polytropic HRS/J viruses [9].

Analytical, diagnostic and therapeutic context of Emv3

  • Using a combination of approaches, including DNA sequencing, peptide mapping, and metabolic labeling of cells with [3H]myristate, we have demonstrated that the defect in Emv-3 most likely results from a single nucleotide substitution within the gene for p15gag that inhibits myristylation of the Pr65gag N terminus [8].
  • The endogenous ecotropic provirus Emv-3 present in DBA/2 mice is poorly expressed in the animal, as well as in cell cultures [5].


  1. Mechanism of chemical activation of expression of the endogenous ecotropic murine leukemia provirus Emv-3. Mercer, J.A., Lee, K.H., Nexø, B.A., Jenkins, N.A., Copeland, N.G. J. Virol. (1990) [Pubmed]
  2. A murine leukemia virus expressed in aged DBA/2 mice is derived by recombination of the Emv-3 locus and another endogenous gag sequence. Bartman, T., Murasko, D.M., Sieck, T.G., Turturro, A., Hart, R., Blank, K.J. Virology (1994) [Pubmed]
  3. Lack of ecotropic virus involvement in induction of lymphomas in DBA/2J mice by 7,12-dimethylbenz(a)anthracene. Mercer, J.A., Jenkins, N.A., Copeland, N.G. J. Virol. (1990) [Pubmed]
  4. A replication-competent, endogenous retrovirus from an aged DBA/2 mouse contains the complete env from Emv-3 and a novel gag partially related to AKT-8. Bartman, T., Murasko, D.M., Blank, K.J. J. Virol. (1995) [Pubmed]
  5. A nucleotide substitution in the gag N terminus of the endogenous ecotropic DBA/2 virus prevents Pr65gag myristylation and virus replication. Jørgensen, E.C., Kjeldgaard, N.O., Pedersen, F.S., Jørgensen, P. J. Virol. (1988) [Pubmed]
  6. Linkage of the mouse LDL receptor gene on chromosome 9. Frank, S.L., Taylor, B.A., Lusis, A.J. Genomics (1989) [Pubmed]
  7. DNAs of two molecularly cloned endogenous ecotropic proviruses are poorly infectious in DNA transfection assays. Copeland, N.G., Bedigian, H.G., Thomas, C.Y., Jenkins, N.A. J. Virol. (1984) [Pubmed]
  8. Poorly expressed endogenous ecotropic provirus of DBA/2 mice encodes a mutant Pr65gag protein that is not myristylated. Copeland, N.G., Jenkins, N.A., Nexø, B., Schultz, A.M., Rein, A., Mikkelsen, T., Jørgensen, P. J. Virol. (1988) [Pubmed]
  9. Role of recombinant ecotropic and polytropic viruses in the development of spontaneous thymic lymphomas in HRS/J mice. Thomas, C.Y., Khiroya, R., Schwartz, R.S., Coffin, J.M. J. Virol. (1984) [Pubmed]
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