Disease relevance of tzs

• Cloning and nucleotide sequence of the tzs gene from Agrobacterium tumefaciens strain T37 [1].
• Nucleotide sequence and expression of a Pseudomonas savastanoi cytokinin biosynthetic gene: homology with Agrobacterium tumefaciens tmr and tzs loci [2].
• A single new labelled protein of 27,200 daltons was detected in Escherichia coli maxicells expressing the cloned tzs gene [1].

High impact information on tzs

• The nopaline-plasmid-specific locus tzs and the octopine-plasmid-specific locus pinF (virH) are not involved in the strain specificity [3].
• Significant sequence homology is found between ptz and both the tzs and tmr genes from Agrobacterium tumefaciens [2].
• The tzs gene is responsible for the secretion of trans-zeatin into bacterial culture medium and in addition has the cytokinin biosynthetic activity, dimethylallylpyrophosphate:AMP dimethylallyltransferase [1].
• The trans-zeatin secretion locus (tzs), from the nopaline Ti plasmid of Agrobacterium tumefaciens strain T37, was cloned and the nucleotide sequence determined [1].
• To study the biosynthetic pathway, the tzs gene, encoding the Tzs (trans-zeatin-synthesizing) protein from A. tumefaciens, was cloned and the protein was overproduced and purified [4].

Biological context of tzs

• Significant sequence homology was observed between the tzs and the published tmr sequence from pTiT37 [1].
• Sequences present in the promoter region of tzs were found to be similar to those present in genes regulated by bacterial two-component positive regulatory systems [5].

Other interactions of tzs

• The relative ability of members of a set of phenols to induce tzs expression was examined and found to be parallel to that reported for activation of other virulence genes [5].
• To test its biological activity, the Tm-4 ipt gene was inserted into a specially constructed, disarmed Ti vector lacking tzs and tested on tobacco, where the rearranged ipt gene induced shoot formation [6].

Analytical, diagnostic and therapeutic context of tzs

• Southern blot analysis showed substantial homology of the Agrobacterium tzs genes to each other but not to the two Pseudomonas genes [7].

References