Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

virE1 - type IV secretion system chaperone VirE1

Agrobacterium fabrum str. C58

Hirooka, T. et al., Jin, S.G. et al., Rogowsky, P.M. et al., Yusibov, V.M. et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of virE1

Characterization of the virE locus of Agrobacterium tumefaciens plasmid pTiC58 [1].
Two different promoters were detected, one which operates in A. tumefaciens and one which operates in Escherichia coli. virE is transcribed from left to right toward the T region [1].

High impact information on virE1

Protoplast infections using an A. tumefaciens virE mutant result in a low level of accumulation of T-strands in the plant cells [2].
In this study we identified a cis-acting regulatory sequence in the 5'-noncoding region of the virE operon that is essential for this activation [3].
Western blot (immunoblot) analysis showed that the 70 base pairs upstream of the transcriptional start site were sufficient for full virE gene induction [3].
The nucleotide sequence of virE revealed three open reading frames, arranged as an operon, with a potential coding capacity for proteins of 9, 7.1, and 63.5 kilodaltons [1].
The promoter region of virE was analyzed by using gene fusions to promoterless cat and lux genes [1].

Chemical compound and disease context of virE1

The virE locus that is responsible for the efficiency of infection by Agrobacterium tumefaciens (T. Hirooka and C. Kado, J. Bacteriol. 168:237-243, 1986) is located next to the right boundary of the virulence (Vir) region of the nopaline plasmid pTiC58 [1].

Biological context of virE1

This locus is very similar to the virE locus of octopine type Ti plasmids on the basis of nucleotide and amino acid sequence comparisons as well as genetic complementation analyses [1].
Two open reading frames not previously characterized, virH and ORF5, were uncovered within the virD/virE intervening spacer region [4].

Associations of virE1 with chemical compounds

In A. tumefaciens, the expression of virE was induced by acetosyringone and required the presence of pTiC58 [1].

References

Characterization of the virE locus of Agrobacterium tumefaciens plasmid pTiC58. Hirooka, T., Rogowsky, P.M., Kado, C.I. J. Bacteriol. (1987) [Pubmed]
Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells. Yusibov, V.M., Steck, T.R., Gupta, V., Gelvin, S.B. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
The regulatory VirG protein specifically binds to a cis-acting regulatory sequence involved in transcriptional activation of Agrobacterium tumefaciens virulence genes. Jin, S.G., Roitsch, T., Christie, P.J., Nester, E.W. J. Bacteriol. (1990) [Pubmed]
Molecular characterization of the vir regulon of Agrobacterium tumefaciens: complete nucleotide sequence and gene organization of the 28.63-kbp regulon cloned as a single unit. Rogowsky, P.M., Powell, B.S., Shirasu, K., Lin, T.S., Morel, P., Zyprian, E.M., Steck, T.R., Kado, C.I. Plasmid (1990) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.