High impact information on Cct4

• The A45 cDNA encodes a predicted polypeptide of M(r) 58,118 that exhibits 32% overall amino acid sequence identity to TCP-1 and contains the putative adenosine triphosphate-binding domain and two characteristic consensus regions that are conserved in all chaperonins [1].
• We have isolated and cloned a novel gene called A45 from a cDNA library constructed from poly (A)+ RNA of a multipotent hematopoietic progenitor clone [1].
• Restriction mapping analysis using YAC and pulse-field gel electrophoresis showed that Cct4 is located within a region about 300 kb from the imprinted gene U2af1-rs1 [2].
• The localization of the human homologue of Cct4 on chromosome 2 corresponds well with the fact that homologues of other genes in the proximal region of mouse chromosome 11 also map to the region of conserved synteny in human chromosome 2 [2].
• Chromosomal assignment and imprinting tests for the mouse delta subunit of the cytosolic chaperonin containing TCP-1 (Cct4) gene to proximal chromosome 11 [2].

Other interactions of Cct4

• A new diagnostic marker based on a polymorphism in the closely linked chaperonine gene Cct4 enabled us to diagnose the allelic status at the wr locus within the original background strain C57BL/6 [3].

Analytical, diagnostic and therapeutic context of Cct4

• CCT delta mRNA was detected in both biosynthetically active (embryonating) and dormant (diapausing) Ae. triseriatus embryos by RT-PCR analysis [4].

References