Disease relevance of iap

• Crude extracts of a multiply peptidase-deficient strain of Salmonella typhimurium contain an aminopeptidase that specifically removes N-terminal methionine from peptides [1].
• Many mutant strains devoid of aminopeptidase activity have been isolated in Escherichia coli [2].
• Comparison of the active sites of peptidase T, carboxypeptidase G2, Aeromonas proteolytica aminopeptidase, carboxypeptidase A and leucine aminopeptidase reveals a common structural framework with interesting similarities and differences in the active sites and in the zinc coordination [3].

High impact information on iap

• We propose to call this enzyme peptidase M (methionine-specific aminopeptidase) [1].
• Innate immunity in the crypt lumen may be modulated by aminopeptidase modification of alpha-defensins after peptide secretion [4].
• Aminopeptidase digestion suggests periplasmic localization of their amino termini [5].
• UMP-kinase might function as the real sensor of the internal pyrimidine nucleotide pool and act in concert with the integration host factor (IHF) and aminopeptidase A (PepA alias CarP and XerB) in the elaboration of the complex nucleoprotein structure required for pyrimidine-specific repression of carP1 promoter activity [6].
• The pancreatic juice from a normal dog had very high gamma-glutamyltransferase (GGT) and aminopeptidase activities and low sulfatase activity, but it had no beta-glucuronidase activity [7].

Biological context of iap

• The pepM gene coding for a methionine-specific aminopeptidase was cloned from Salmonella typhimurium and its nucleotide sequence determined [8].

Anatomical context of iap

• Their topology in the cytoplasmic membrane was determined by cleavage with aminopeptidase K, proteinase K, and trypsin in spheroplasts and cell lysates [9].

Associations of iap with chemical compounds

• The aminopeptidase activity of CarP (PepA) was found to be dispensable for its role in pyrimidine-mediated repression of carAB transcription [10].
• The action of this combination involved both an inhibitory and a bacteriolytic mechanism which was abolished by concurrent treatment with the aminopeptidase inhibitor, bestatin [11].

References