Disease relevance of mnt

• These phages, derivatives of Salmonella phage P22 that have substitutions of synthetic, symmetric trp operators for the P22 mnt operator, provide a genetic assay for DNA binding in vivo [1].
• Previous work has shown that the sieA gene of Salmonella bacteriophage P22 is located between the genes mnt and 16 [2].

High impact information on mnt

• Four independent mutations change the same CAC codon in the mnt gene to CCT or CCC [3].
• The arc and mnt genes of bacteriophage P22 encode small repressor proteins [4].
• The isolation and characterization of streptozotocin (STZ)-induced mutations in the phage P22 mnt repressor gene is described [5].
• Our results indicate that one of the repressors required for maintenance of lysogeny, the mnt gene product, may be partially responsible for this phenomenon [6].
• This region includes the ant gene, which encodes the P22 antirepressor protein, and the mnt and arc genes, which encode proteins that negatively regulate antirepressor synthesis [7].

Chemical compound and disease context of mnt

• Analysis of forward mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine in the bacteriophage P22 mnt repressor gene [8].

Biological context of mnt

• The arc and ant genes are transcribed rightward from the Pant promoter, while mnt is transcribed leftward from a promotor that may overlap Pant [7].
• The majority of these mutations are A:T----G:C transitions that occur within six base pairs of the two 5'-GATC-3' sequences in the mnt gene [9].
• We describe the isolation and genetic characterization of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced mutations in the phage P22 mnt repressor gene cloned in plasmid pBR322 [8].
• The mnt gene may be a useful target for determining mutagenic specificity at the nucleotide level because forward mutations are easily isolated, the target size is small, and the DNA sequence changes of mutations can be determined rapidly [8].
• Mutations in the mnt repressor gene or its operator on this plasmid, pPY98, confer a tetracycline resistance phenotype, whereas the wild-type plasmid confers tetracycline sensitivity [8].

References