Disease relevance of tra

• Mutational analysis of the tra locus of the broad-host-range Streptomyces plasmid pIJ101 [1].
• Promoter-distal region of the tra operon of F-like sex factor R100 in Escherichia coli K-12 [2].

High impact information on tra

• With pOX38-tra715 or its derivatives, we were able to label specifically the products of tra genes located throughout the long tra operon, by using rifampicin [3].
• Efficient interbacterial transfer of streptomycete plasmid pIJ101 requires the pIJ101 tra gene, as well as a cis-acting plasmid function known as clt [4].
• The tra region of the nopaline-type Ti plasmid is a chimera with elements related to the transfer systems of RSF1010, RP4, and F [5].
• By using transposon mutagenesis with Tn3HoHo1, we localized the tra determinants to an 8.5-kb region that also contains the oriT region [5].
• We determined the complete nucleotide sequence of an 8,755-bp region of the Ti plasmid encompassing the transposon insertions defining tra [5].

Biological context of tra

• The transposon Tn5 (which confers kanamycin resistance) was inserted at different positions into pSM6, and the transposition derivatives were tested for ability to complement defined tra mutants of the F sex factor [2].
• The tra gene product (Tra) is a membrane protein, a portion of which shows similarity to transfer proteins of other streptomycete plasmids as well as additional bacterial chromosome partitioning proteins [6].
• Plasmid pIJ101 from Streptomyces lividans encodes a single gene, tra, that is essential for both plasmid transfer and mobilization of chromosomes during mating [6].
• We show here that mutations in translational open reading frames (ORFs) that map within the kor loci abolish repression of the S. lividans lac gene directed by the tra and kilB promoters [7].

References