Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

BgdVgp1 - Orf2

Blattella germanica densovirus 1

Afanasiev, B.N. et al., Mukha, D.V. et al., Cheng, L. et al., Wang, J. et al., Giraud, C. et al., et al.

Mukha, Chumachenko, Dykstra, Kurtti, Schal,

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of BgdVgp1

These included a trypsin-like protease, a zinc-finger protein, regulator of chromosome condensation proteins, a densovirus capsid-like protein and a phosphotransferase [1].
Virions, about 22 nm in diameter, had a buoyant density of 1.43 g/cm3 and contained three capsid polypeptides with molecular weights of 53, 41 and 40 kDa [2].
The second one related to the Parvoviridae family, is a 25 nm in diameter icosahedral virus with a DNA genome and a capsid constituted of 4 polypeptides of respectively, 90,000; 64,000; 56,000 and 43,500 daltons [3].

High impact information on BgdVgp1

Five open reading frames (ORFs) were detected in the 5335 nt sequence: two ORFS that were on one DNA strand encoded structural capsid proteins (69.7 and 24.8 kDa) and three ORFs that were on the other strand encoded non-structural proteins (60.2, 30.3 and 25.9 kDa) [4].
Deletions in inverted terminal repeats or in NS functions did not abolish expression of capsid polypeptides but reduced it dramatically [5].
When these recombinant constructs were transfected into Aedes albopictus C6/36 cells, the beta-gal was expressed efficiently from the right ORF (encoding capsid proteins, Vps) and the mid ORF (encoding putative nonstructural protein 2) [6].
The recombinant AeDNV genomes (carrying the beta-gal gene) supplied with the AeDNV capsid proteins can be packaged into infectious transducing particles [6].

Chemical compound and disease context of BgdVgp1

Three-dimensional structure determination of capsid of Aedes albopictus C6/36 cell densovirus [7].

Biological context of BgdVgp1

The 'plus' strand of the genome contains three large open reading frames (ORFs), the left and the mid-ORFs (within the left ORF) in the left-half encoding the non-structural proteins and the right ORF in the right-half encoding viral capsid proteins [8].

Analytical, diagnostic and therapeutic context of BgdVgp1

Western blot analysis revealed that beta-gal was expressed as chimeric capsid-beta-gal polypeptides [5].
The three-dimensional structure of capsid of Aedes albopictus C6/36 densovirus was determined to 14-A resolution by electron cryomicroscopy and computer reconstruction [7].

References

Genomic comparison of Neodiprion sertifer and Neodiprion lecontei nucleopolyhedroviruses and identification of potential hymenopteran baculovirus-specific open reading frames. Lauzon, H.A., Garcia-Maruniak, A., Zanotto, P.M., Clemente, J.C., Herniou, E.A., Lucarotti, C.J., Arif, B.M., Maruniak, J.E. J. Gen. Virol. (2006) [Pubmed]
A parvo-like virus persistently infecting a C6/36 clone of Aedes albopictus mosquito cell line and pathogenic for Aedes aegypti larvae. Jousset, F.X., Barreau, C., Boublik, Y., Cornet, M. Virus Res. (1993) [Pubmed]
Evidence for two small viruses persistently infecting established cell lines of Phthorimaea operculella, deriving from embryos of the potato tuber moth. Lery, X., Zeddam, J.L., Giannotti, J., Abol-Ela, S. New Microbiol. (1998) [Pubmed]
Characterization of a new densovirus infecting the German cockroach, Blattella germanica. Mukha, D.V., Chumachenko, A.G., Dykstra, M.J., Kurtti, T.J., Schal, C. J. Gen. Virol. (2006) [Pubmed]
The densovirus of Junonia coenia (Jc DNV) as an insect cell expression vector. Giraud, C., Devauchelle, G., Bergoin, M. Virology (1992) [Pubmed]
Densovirus of Aedes aegypti as an expression vector in mosquito cells. Afanasiev, B.N., Kozlov, Y.V., Carlson, J.O., Beaty, B.J. Exp. Parasitol. (1994) [Pubmed]
Three-dimensional structure determination of capsid of Aedes albopictus C6/36 cell densovirus. Cheng, L., Chen, S., Brannan, J.M., Jakana, J., Zhang, Q., Zhou, Z.H., Zhang, J. Sci. China, C, Life Sci. (2004) [Pubmed]
Nucleotide sequence and genomic organization of a newly isolated densovirus infecting Dendrolimus punctatus. Wang, J., Zhang, J., Jiang, H., Liu, C., Yi, F., Hu, Y. J. Gen. Virol. (2005) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.