High impact information on Tcea2

• SII-T1 is a tissue-specific member of the transcription elongation factor S-II that is expressed specifically in male germ cells [1].
• Also, transactivation by GRIP1tau was stimulated by SII-T1 in a dose-dependent manner [1].
• We have identified testis-specific SII (SII-T1) and shown that it was expressed exclusively in spermatocytes [2].
• Here, we isolated a 335-bp fragment of the cDNA for mouse SII-T1, and used it to examine the expression of the SII-T1 gene in the testis by in situ hybridization [3].
• RT-PCR experiments using testis RNA from W/Wv mutant mice also suggested that SII-T1 is a specific transcription elongation factor essential for spermatogenesis [3].

Biological context of Tcea2

• Gene organization and chromosome mapping of the testis-specific S-II [4].
• These results indicate that expression of mouse S-II-T1 is restricted to testicular germ cells during and after meiosis in the course of spermatogenesis [5].

Anatomical context of Tcea2

• The results indicated that the SII-T1 gene is expressed exclusively in spermatocytes, showing no appreciable expression in spermatogonia, spermatids, or Leydig cells [3].

Analytical, diagnostic and therapeutic context of Tcea2

• The transactivation domain of GRIP1tau overlapped with the region essential for interaction with SII-T1, as revealed by co-immunoprecipitation assays [1].

References