High impact information on ALDH1A3

• Retinoic acid biosynthesis by normal human breast epithelium is via aldehyde dehydrogenase 6, absent in MCF-7 cells [1].
• Identification of ALDH6 as the retinaldehyde dehydrogenase present in normal human breast epithelia provides the first tool necessary for studying the loss of RA synthetic ability in cancer cells and the relationship of this process to malignant transformation [1].
• When MCF-7 cells were then transfected with ALDH6, they (re)gained the ability to oxidize retinal to RA as well as some ability to synthesize RA when provided with retinol [1].
• During the first postnatal week, the RALDH3-expressing territory translocates in the caudal cortex from the medial limbic lobe to the adjacent neocortex [2].
• In this paper, we describe the cloning, characterization, and chromosomal mapping of an aldehyde dehydrogenase gene (ALDH6) expressed in the human salivary gland [3].

Biological context of ALDH1A3

• The human ALDH6 gene spans about 37 kb and consists of 13 exons [3].
• The cloned ALDH6 cDNA is 3457 bp in length and contains an open reading frame encoding 512 amino acid residues [3].
• The ALDH6 gene was assigned to chromosome 15q26 using fluorescence in situ hybridization [3].
• The deduced amino acid sequence showed that ALDH6 is larger than the human liver ALDH1 by 11 amino acid residues at the N-terminal, and the degree of identity between the two isozymes is 70% with an alignment of 500 amino acid residues [3].

Other interactions of ALDH1A3

• Furthermore, marker D15S642, not far from the ALDH6 gene, provided an MC p-value of 0.0005 in ethnic groups "White, Hispanic" and "White, non-Hispanic."[4]

Analytical, diagnostic and therapeutic context of ALDH1A3

• Northern blot analysis demonstrated that the ALDH6 gene is expressed at low levels in many tissues and at higher levels in salivary gland, stomach, and kidney [3].
• Molecular cloning, genomic organization, and chromosomal localization of an additional human aldehyde dehydrogenase gene, ALDH6 [3].

References