Disease relevance of SEPHS2

• In the present study, Sps1 and Sps2 were cloned from a cDNA library prepared from human lung adenocarcinoma cells (NCIH441) [1].

High impact information on SEPHS2

• Selenophosphate synthetase genes from lung adenocarcinoma cells: Sps1 for recycling L-selenocysteine and Sps2 for selenite assimilation [1].
• These results suggest the existence of an autoregulatory mechanism involving the incorporation of Sec into SPS2 that might be relevant to blood cell biology [2].
• In addition, we show that Sps2 mRNA is up-regulated upon activation of T lymphocytes and have mapped the Sps2 gene to mouse chromosome 7 [2].
• Sps2 mRNA levels are elevated in organs previously implicated in the synthesis of selenoproteins and in active sites of blood cell development [2].
• Using the mouse gene isolated from the hematopoietic cell line FDCPmixA4, we devised a construct for protein expression that results in the insertion of a FLAG tag sequence at the N terminus of the SPS2 protein [2].

Associations of SEPHS2 with chemical compounds

• Addition of tetra-N-methylammonium cation as ion-pairing agent to a methanol-sulfuric acid-water eluent increases the retention time of the anionic SPS2- on a C18 column sufficiently to separate this compound from Cu(II) ion and additive by-products [3].
• Selenoproteins with known functions identified so far include five glutathione peroxidases, two deiodinases, several thioredoxin reductases, and selenophosphate synthetase 2 [4].

References