The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

SEPHS1  -  selenophosphate synthetase 1

Homo sapiens

Synonyms: SELD, SPS, SPS1, Selenide, water dikinase 1, Selenium donor protein 1, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of SEPHS1

  • Expression of the recombinant plasmids containing Sps1 or Sps2Cys was highly toxic to Escherichia coli host cells grown aerobically [1].
  • In the present study, Sps1 and Sps2 were cloned from a cDNA library prepared from human lung adenocarcinoma cells (NCIH441) [1].
  • Biosynthesis of sucrose-6-P catalyzed by sucrose-phosphate synthase (SPS), and the presence of sucrose-phosphate phosphatase (SPP) leading to the formation of sucrose, have both been ascertained in a prokaryotic organism: Anabaena 7119, a filamentous heterocystic cyanobacterium [2].
  • We report on the isolation and characterization of cDNA clones encoding SPS from Craterostigma plantagineum Hochst., a resurrection plant in which the accumulation of sucrose is considered to play an important role in tolerance to severe protoplastic dehydration [3].
  • Significant reductions were noted in all major seizure types treatable with resective surgery; complex partial (CPS), simple partial (SPS), and secondarily generalized tonic-clonic seizures (GTC) (all p < 0.05) [4].

Psychiatry related information on SEPHS1

  • The Self-Rating Scale (SPS) measure of self-esteem, Sensation-Seeking Scale Form II (SSS), Leisure Boredom Scale (LBS), and a time-use inventory yielded quantitative data [5].
  • For male Ss, verbal reinforcement increased self-disclosure relative to the interviewer seld-disclosure condition [6].
  • Assessment devices such as the Social Interaction Anxiety Scale, the Social Phobia Scale (SIAS and SPS; Behav. Res. Therapy, 36 (4) (1998) 455), and the Anxiety Sensitivity Index (ASI; Behav. Res. Therapy, 24 (1986) 1) are good measures of the presumably separate lower-order factors [7].

High impact information on SEPHS1


Chemical compound and disease context of SEPHS1

  • Selenophosphate synthetase genes from lung adenocarcinoma cells: Sps1 for recycling L-selenocysteine and Sps2 for selenite assimilation [1].
  • The results suggest that the increased remobilization of carbon reserves by water stress is attributable to the enhanced FEH and SPS activities in wheat stems, and that ABA plays a vital role in the regulation of the key enzymes involved in fructan and sucrose metabolism [11].
  • We tested three blood culture systems used for fastidious organisms: brucella broth with SPS and CO2 (Becton Dickinson), biphasic brain heart infusion agar or broth (Becton Dickinson), and supplemented peptone broth (Vacutainer) [12].
  • When sucrose-phosphate synthase (SPS; EC is expressed in tomato (Lycopersicon esculentum Mill.) from a ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) small subunit (rbcS) promoter, yields are often unchanged but when SPS is expressed from a Cauliflower Mosaic Virus 35S promoter, yield is enhanced up to 80% [13].
  • Using an SPS device (Sandoz Pharma, Basel, Switzerland), monitoring was carried out in 10 male patients with mild essential hypertension (1) after a placebo period, (2) after six months, and (3) after 12 to 13 months of treatment with isradipine (average dose 2.5 mg twice daily) [14].

Biological context of SEPHS1

  • Our results demonstrate that the Asi proteins ensure the fidelity of SPS sensor signaling by maintaining the dormant, or repressed state, of gene expression in the absence of inducing signals [15].
  • Analysis of the carboxyl terminus of SRP 72 has identified a putative cleavage site (SELD/A) for group III caspases, and carboxyl-terminal serine residues that are highly conserved in phylogeny [16].
  • Here we focused on two event-related potential components: the SPS/P600, related to syntactic violation and reanalysis, and the N400 component, related to semantic integration problems [17].
  • Therefore, at least part of the osmotic stress activation of SPS in dark leaves results from phosphorylation of serine-424 catalyzed by a Ca(2+)-dependent, 150-kD protein kinase [18].
  • Analysis of expressed sequence tags indicated similar expression patterns to wheat for each SPS gene family in barley (Hordeum vulgare) but not in more distantly related grasses [19].

Anatomical context of SEPHS1

  • Additionally, it was observed that the expression of the sps1 promoter is regulated by light and dependent on plastid development in photosynthetic tissues, whereas expression in scutellum is independent of both light and plastid development [20].
  • Sps1 cell lines displayed decreased level of reactive oxygen species (ROS) with concomitant increase of certain redox enzymes [21].
  • A human umbilical endothelial cell line, ECV 304, was stably transfected with the genes for both PHGPx and selenophosphate synthetase (selD), which provides selenophosphate for selenoprotein biosynthesis [22].
  • Although all stages were present in all breeding groups, early stages of spermatozoa (SPG I and SPG II) were greatest in postbreeding males, while late stages (SPT and SPS) were highest in prebreeding, calling, and amplexing males [23].
  • Analogous cell interactions were observed with PAH/poly(methacrylic acid) (PAH/PMA), PAH/sulfonated poly(styrene) (PAH/SPS), and poly(diallyldimethylammonium chloride)/SPS (PDAC/SPS) systems, thereby suggesting a general trend in the fibroblasts' response to multilayers [24].

Associations of SEPHS1 with chemical compounds

  • A labile selenium donor compound monoselenophosphate is synthesized from selenide and ATP by selenophosphate synthetase (SPS) [1].
  • In contrast, only a weak complementation of the selD mutant by the Sps1 gene was observed when cells were grown in selenite media [1].
  • Quantitative analysis of the brightener component bis (sodium-sulfopropyl) disulfide (SPS) in acidic copper plating baths poses a real challenge due to the complex chemical matrix containing large amounts of Cu(II) ion and sulfuric acid together with other organic additives and additive decomposition products [25].
  • In dicotyledonous plants there are three SPS gene families: A, B, and C. Here we report the finding of five families of SPS genes in wheat (Triticum aestivum) and other monocotyledonous plants from the family Poaceae (grasses) [19].
  • Feeding okadaic acid to leaves decreased the SPS activation state in the dark and light and in leaves fed mannose [26].

Other interactions of SEPHS1


Analytical, diagnostic and therapeutic context of SEPHS1

  • To explore the mechanisms that regulate the tissue-specific and developmental distribution of SPS, the expression pattern of rice (Oryza sativa) sps1 (GenBank accession no. U33175) was examined by in situ reverse transcriptase-polymerase chain reaction and the expression directed by the sps1 promoter using the beta-glucuronidase reporter gene [20].
  • A 2584 bp long partial cDNA clone encoding SPS was isolated from ripening kiwifruit. cDNA fragments encoding the 5' end were isolated by PCR, and sequencing revealed at least four closely related (> 96% identity) mRNAs expressed early in kiwifruit ripening [28].
  • Southern blot analysis showed that SPS is encoded by a single-copy gene in the rice genome [29].
  • We report an Mg2+-dependent interaction between spinach leaf sucrose-phosphate synthase (SPS) and endogenous 14-3-3 proteins, as evidenced by co-elution during gel filtration and co-immunoprecipitation [30].
  • A synthetic phosphopeptide based on Ser-229 was shown by surface plasmon resonance to bind a recombinant plant 14-3-3, and addition of the phosphorylated SPS-229 peptide was found to stimulate the SPS activity of an SPS:14-3-3 complex [30].


  1. Selenophosphate synthetase genes from lung adenocarcinoma cells: Sps1 for recycling L-selenocysteine and Sps2 for selenite assimilation. Tamura, T., Yamamoto, S., Takahata, M., Sakaguchi, H., Tanaka, H., Stadtman, T.C., Inagaki, K. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  2. Sucrose biosynthesis in a prokaryotic organism: Presence of two sucrose-phosphate synthases in Anabaena with remarkable differences compared with the plant enzymes. Porchia, A.C., Salerno, G.L. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  3. Analysis of cDNA clones encoding sucrose-phosphate synthase in relation to sugar interconversions associated with dehydration in the resurrection plant Craterostigma plantagineum Hochst. Ingram, J., Chandler, J.W., Gallagher, L., Salamini, F., Bartels, D. Plant Physiol. (1997) [Pubmed]
  4. Surgical treatment for partial epilepsy among Norwegian adults. Guldvog, B., Løyning, Y., Hauglie-Hanssen, E., Flood, S., Bjørnaes, H. Epilepsia (1994) [Pubmed]
  5. Urban-rural differences in adolescent self-esteem, leisure boredom, and sensation-seeking as predictors of leisure-time usage and satisfaction. Gordon, W.R., Caltabiano, M.L. Adolescence. (1996) [Pubmed]
  6. The influence of interviewer self-disclosure and verbal reinforcement on personality tests. Little, B.R., Arlett, C., Best, J.A. Journal of clinical psychology. (1976) [Pubmed]
  7. Predicting anxious response to a social challenge: the predictive utility of the social interaction anxiety scale and the social phobia scale in a college population. Gore, K.L., Carter, M.M., Parker, S. Behaviour research and therapy. (2002) [Pubmed]
  8. Selenocysteine. Stadtman, T.C. Annu. Rev. Biochem. (1996) [Pubmed]
  9. Expression of a maize sucrose phosphate synthase in tomato alters leaf carbohydrate partitioning. Worrell, A.C., Bruneau, J.M., Summerfelt, K., Boersig, M., Voelker, T.A. Plant Cell (1991) [Pubmed]
  10. Characterization of potential selenium-binding proteins in the selenophosphate synthetase system. Ogasawara, Y., Lacourciere, G.M., Ishii, K., Stadtman, T.C. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  11. Activities of fructan- and sucrose-metabolizing enzymes in wheat stems subjected to water stress during grain filling. Yang, J., Zhang, J., Wang, Z., Zhu, Q., Liu, L. Planta (2004) [Pubmed]
  12. Evaluation of three commercially available blood culture systems for cultivation of Helicobacter pylori. Kehler, E.G., Midkiff, B.R., Westblom, T.U. J. Clin. Microbiol. (1994) [Pubmed]
  13. Promoter strength and tissue specificity effects on growth of tomato plants transformed with maize sucrose-phosphate synthase. Laporte, M.M., Galagan, J.A., Prasch, A.L., Vanderveer, P.J., Hanson, D.T., Shewmaker, C.K., Sharkey, T.D. Planta (2001) [Pubmed]
  14. Effect of isradipine on 24-h blood pressure profile demonstrated by repeated monitoring. Cihák, R., Widimský, J., Lefflerová, K., Janský, P. Am. J. Hypertens. (1991) [Pubmed]
  15. Inner nuclear membrane proteins asi1, asi2, and asi3 function in concert to maintain the latent properties of transcription factors stp1 and stp2. Zargari, A., Boban, M., Heessen, S., Andr??asson, C., Thyberg, J., Ljungdahl, P.O. J. Biol. Chem. (2007) [Pubmed]
  16. The 72-kDa component of signal recognition particle is cleaved during apoptosis. Utz, P.J., Hottelet, M., Le, T.M., Kim, S.J., Geiger, M.E., van Venrooij, W.J., Anderson, P. J. Biol. Chem. (1998) [Pubmed]
  17. Pronominal reference in sentences about persons or things: an electrophysiological approach. Hammer, A., Jansma, B.M., Lamers, M., Münte, T.F. Journal of cognitive neuroscience. (2005) [Pubmed]
  18. Protein phosphorylation as a mechanism for osmotic-stress activation of sucrose-phosphate synthase in spinach leaves. Toroser, D., Huber, S.C. Plant Physiol. (1997) [Pubmed]
  19. Evolution and function of the sucrose-phosphate synthase gene families in wheat and other grasses. Castleden, C.K., Aoki, N., Gillespie, V.J., MacRae, E.A., Quick, W.P., Buchner, P., Foyer, C.H., Furbank, R.T., Lunn, J.E. Plant Physiol. (2004) [Pubmed]
  20. Tissue-specific and developmental pattern of expression of the rice sps1 gene. Chávez-Bárcenas, A.T., Valdez-Alarcón, J.J., Martínez-Trujillo, M., Chen, L., Xoconostle-Cázares, B., Lucas, W.J., Herrera-Estrella, L. Plant Physiol. (2000) [Pubmed]
  21. p53-Mediated enhancement of radiosensitivity by selenophosphate synthetase 1 overexpression. Chung, H.J., Yoon, S.I., Shin, S.H., Koh, Y.A., Lee, S.J., Lee, Y.S., Bae, S. J. Cell. Physiol. (2006) [Pubmed]
  22. Interleukin-1-induced nuclear factor kappa B activation is inhibited by overexpression of phospholipid hydroperoxide glutathione peroxidase in a human endothelial cell line. Brigelius-Flohé, R., Friedrichs, B., Maurer, S., Schultz, M., Streicher, R. Biochem. J. (1997) [Pubmed]
  23. Reproductive endocrinology of the explosively breeding desert spadefoot toad, Scaphiopus couchii. Harvey, L.A., Propper, C.R., Woodley, S.K., Moore, M.C. Gen. Comp. Endocrinol. (1997) [Pubmed]
  24. Rational design of cytophilic and cytophobic polyelectrolyte multilayer thin films. Mendelsohn, J.D., Yang, S.Y., Hiller, J., Hochbaum, A.I., Rubner, M.F. Biomacromolecules (2003) [Pubmed]
  25. Ion-pair chromatography of bis (sodium-sulfopropyl) disulfide brightener in acidic copper plating baths. Palmans, R., Claes, S., Vanatta, L.E., Coleman, D.E. Journal of chromatography. A. (2005) [Pubmed]
  26. Site-specific serine phosphorylation of spinach leaf sucrose-phosphate synthase. Huber, J.L., Huber, S.C. Biochem. J. (1992) [Pubmed]
  27. The facts and controversies about selenium. Dodig, S., Cepelak, I. Acta pharmaceutica (Zagreb, Croatia) (2004) [Pubmed]
  28. Sucrose-phosphate synthase steady-state mRNA increases in ripening kiwifruit. Langenkämper, G., McHale, R., Gardner, R.C., MacRae, E. Plant Mol. Biol. (1998) [Pubmed]
  29. Characterization of a rice sucrose-phosphate synthase-encoding gene. Valdez-Alarcón, J.J., Ferrando, M., Salerno, G., Jimenez-Moraila, B., Herrera-Estrella, L. Gene (1996) [Pubmed]
  30. Site-specific regulatory interaction between spinach leaf sucrose-phosphate synthase and 14-3-3 proteins. Toroser, D., Athwal, G.S., Huber, S.C. FEBS Lett. (1998) [Pubmed]
WikiGenes - Universities