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Gene Review:

CHST5 - carbohydrate (N-acetylglucosamine 6-O)...

Homo sapiens

Synonyms: Carbohydrate sulfotransferase 5, FLJ22167, GST4-alpha, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-alpha, GlcNAc6ST-3, ...

Hayashida, Y. et al., Lee, J.K. et al., Bartes, A. et al., Hemmerich, S. et al., Seko, A. et al.

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Disease relevance of CHST5

Expression levels of transcript for GlcNAc6ST-3 in normal mucosa were significantly higher than those in adenocarcinomas [1].

High impact information on CHST5

To ascertain the importance of the carbohydrate chain sulfation step in KS functionality, we generated a strain of mice with a targeted gene deletion in Chst5, which encodes an N-acetylglucosamine-6-O-sulfotransferase that is integral to the sulfation of KS chains [2].
The corneal stroma of the Chst5-null mouse exhibited widespread structural alterations in collagen fibrillar architecture, including decreased interfibrillar spacing and a more spatially disorganized collagen array [2].
Histochemically stained KSPGs coassociated with fibrillar collagen in WT corneas, but were not identified in the Chst5-null tissue [2].
Conversely, abnormally large chondroitin sulfate/dermatan sulfate PG complexes were abundant throughout the Chst5-deficient cornea, indicating an alteration of controlled PG production in the mutant cornea [2].
In contrast to GlcNAc6ST-2, GlcNAc6ST-3 transcripts are present minimally, if at all, in HECs; moreover, this enzyme is not able to generate the 6-sulfo sLex epitope in transfected cells [3].

Biological context of CHST5

The human GST4 locus actually contains two highly similar open reading frames (ORF) that are 50 kb apart and encode two highly similar enzyme isoforms termed GST-4 alpha and GST-4 beta [4].

Anatomical context of CHST5

PCR analysis of cDNAs derived from a panel of tissues and purified cell populations confirms the strong expression of GlcNAc6ST-3 in gut-associated tissues and extends the expression to include lymphocytes [3].

Associations of CHST5 with chemical compounds

Intestinal N-acetylglucosamine 6-O-sulfotransferase (I-GlcNAc6ST, GST-4alpha) and corneal N-acetylglucosamine 6-O-sulfotransferases (C-GlcNAc6ST, GST-4beta) are two highly homologous GlcNAc 6-O-sulfotransferase isozymes encoded by two intronless open reading frames that reside approximately 50 kb apart on human chromosome 16q23 [5].

Other interactions of CHST5

Activities and expression pattern of the carbohydrate sulfotransferase GlcNAc6ST-3 (I-GlcNAc6ST): functional implications [3].
Limited information has been available about the closely related sulfotransferase known as GlcNAc6ST-3 (I-GlcNAc6ST) [3].

References

Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma. Seko, A., Nagata, K., Yonezawa, S., Yamashita, K. Glycobiology (2002) [Pubmed]
Matrix morphogenesis in cornea is mediated by the modification of keratan sulfate by GlcNAc 6-O-sulfotransferase. Hayashida, Y., Akama, T.O., Beecher, N., Lewis, P., Young, R.D., Meek, K.M., Kerr, B., Hughes, C.E., Caterson, B., Tanigami, A., Nakayama, J., Fukada, M.N., Tano, Y., Nishida, K., Quantock, A.J. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
Activities and expression pattern of the carbohydrate sulfotransferase GlcNAc6ST-3 (I-GlcNAc6ST): functional implications. Lee, J.K., Bistrup, A., van Zante, A., Rosen, S.D. Glycobiology (2003) [Pubmed]
Chromosomal localization and genomic organization for the galactose/ N-acetylgalactosamine/N-acetylglucosamine 6-O-sulfotransferase gene family. Hemmerich, S., Lee, J.K., Bhakta, S., Bistrup, A., Ruddle, N.R., Rosen, S.D. Glycobiology (2001) [Pubmed]
Sulfation of endothelial mucin by corneal keratan N-acetylglucosamine 6-O-sulfotransferase (GST-4beta). Bartes, A., Bhakta, S., Hemmerich, S. Biochem. Biophys. Res. Commun. (2001) [Pubmed]

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