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Gene Review

CHST4  -  carbohydrate (N-acetylglucosamine 6-O)...

Homo sapiens

Synonyms: Carbohydrate sulfotransferase 4, GST-3, GST3, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3, GlcNAc6ST-2, ...
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Disease relevance of CHST4


High impact information on CHST4


Chemical compound and disease context of CHST4


Biological context of CHST4


Anatomical context of CHST4


Associations of CHST4 with chemical compounds

  • The ability of GlcNAc6ST-1 to sulfate core 2 structure as efficiently as GlcNAc6ST-2 is consistent with the view that GlcNAc6ST-1 is also involved in the synthesis of l-selectin ligand [1].
  • CHST3, CHST4 and TPST2 mRNA levels after exposure to 50 microM NO-1886 were not significantly increased [16].
  • I50 values for Cibacron blue, bromosulphophthalein, indocyanine green, hematin and ethacrynic acid were 0.76 microM, 47.9 microM, 7.59 microM, 0.03 microM and 0.79 microM for GST2, and 0.479 microM, 79.4 microM, 89.1 microM, 32.4 microM and 1.15 microM for GST3, respectively [17].
  • The sulfation of these ligands depends on the action of two HEV-expressed N-acetylglucosamine 6-O-sulfotransferases: GlcNAc6ST-2 and to a lesser degree GlcNAc6ST-1 [18].
  • The assay proceeds by transfer of 35S-labeled sulfate from [35S]-3(')-phosphoadenosine-5(')-phosphosulfate (PAPS) to the free amino groups of de-N-sulfated heparin (NDST-1), or the 6-hydroxyl groups of N-acetylglucosamine residues linked to a polyacrylamide scaffold (HEC-GlcNAc-6-ST) [19].

Regulatory relationships of CHST4


Other interactions of CHST4

  • Both GlcNAc6ST-1 and GlcNAc6ST-2 efficiently utilized the following oligosaccharide structures as acceptors: GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe, and GlcNAcbeta1-2Man [1].
  • Here we describe an efficient dot-blot assay for high-throughput screening of two enzymes, heparan sulfate N-deacetylase/N-sulfotransferase (NDST-1) and high-endothelial cell GlcNAc-6-sulfotransferase (HEC-GlcNAc-6-ST) [19].

Analytical, diagnostic and therapeutic context of CHST4

  • We showed previously by enzymological characterization and RT-PCR that colonic mucinous adenocarcinoma tissues ectopically express GlcNAc6ST-2, a member of the carbohydrate 6-O-sulfotransferase family (Seko, A. et al. (2002) Glycobiology 12, 379-388) [3].
  • In addition, the original sample used to develop the LSST consisted of symptomatic athletes only, with no asymptomatic control group [20].


  1. Specificities of N-acetylglucosamine-6-O-sulfotransferases in relation to L-selectin ligand synthesis and tumor-associated enzyme expression. Uchimura, K., El-Fasakhany, F.M., Hori, M., Hemmerich, S., Blink, S.E., Kansas, G.S., Kanamori, A., Kumamoto, K., Kannagi, R., Muramatsu, T. J. Biol. Chem. (2002) [Pubmed]
  2. Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma. Seko, A., Nagata, K., Yonezawa, S., Yamashita, K. Glycobiology (2002) [Pubmed]
  3. Ectopic expression of N-acetylglucosamine 6-O-sulfotransferase 2 in chemotherapy-resistant ovarian adenocarcinomas. Kanoh, A., Seko, A., Ideo, H., Yoshida, M., Nomoto, M., Yonezawa, S., Sakamoto, M., Kannagi, R., Yamashita, K. Glycoconj. J. (2006) [Pubmed]
  4. A HEV-restricted sulfotransferase is expressed in rheumatoid arthritis synovium and is induced by lymphotoxin-alpha/beta and TNF-alpha in cultured endothelial cells. Pablos, J.L., Santiago, B., Tsay, D., Singer, M.S., Palao, G., Galindo, M., Rosen, S.D. BMC Immunol. (2005) [Pubmed]
  5. A small-molecule switch for Golgi sulfotransferases. de Graffenried, C.L., Laughlin, S.T., Kohler, J.J., Bertozzi, C.R. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  6. Identification of cutaneous lymphocyte-associated antigen as sialyl 6-sulfo Lewis X, a selectin ligand expressed on a subset of skin-homing helper memory T cells. Ohmori, K., Fukui, F., Kiso, M., Imai, T., Yoshie, O., Hasegawa, H., Matsushima, K., Kannagi, R. Blood (2006) [Pubmed]
  7. Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. Konohana, A., Konohana, I., Schroeder, W.T., O'Brien, W.R., Amagai, M., Greer, J., Shimizu, N., Gammon, W.R., Siciliano, M.J., Duvic, M. J. Invest. Dermatol. (1990) [Pubmed]
  8. Activities and expression pattern of the carbohydrate sulfotransferase GlcNAc6ST-3 (I-GlcNAc6ST): functional implications. Lee, J.K., Bistrup, A., van Zante, A., Rosen, S.D. Glycobiology (2003) [Pubmed]
  9. Golgi localization of carbohydrate sulfotransferases is a determinant of L-selectin ligand biosynthesis. de Graffenried, C.L., Bertozzi, C.R. J. Biol. Chem. (2003) [Pubmed]
  10. The human glutathione S-transferases: studies on the tissue distribution and genetic variation of the GST1, GST2 and GST3 isozymes. Strange, R.C., Faulder, C.G., Davis, B.A., Hume, R., Brown, J.A., Cotton, W., Hopkinson, D.A. Ann. Hum. Genet. (1984) [Pubmed]
  11. BamHI restriction fragment length polymorphism (RFLP) at the human GST3 gene locus. Kumar, A., Das, B.C., Sharma, J.K. Hum. Genet. (1994) [Pubmed]
  12. Isolation of a cDNA clone and localization of the human glutathione S-transferase 3 genes to chromosome bands 11q13 and 12q13-14. Board, P.G., Webb, G.C., Coggan, M. Ann. Hum. Genet. (1989) [Pubmed]
  13. Sulfated L-selectin ligands as a therapeutic target in chronic inflammation. Uchimura, K., Rosen, S.D. Trends Immunol. (2006) [Pubmed]
  14. Immunohistochemical localization of human liver glutathione S-transferase (GST) isozymes with special reference to polymorphic GST1. Abei, M., Harada, S., Tanaka, N., McNeil, M., Osuga, T. Biochim. Biophys. Acta (1989) [Pubmed]
  15. Roles of sulfated glycans in lymphocyte homing. Kawashima, H. Biol. Pharm. Bull. (2006) [Pubmed]
  16. Effects of NO-1886 (Ibrolipim), a lipoprotein lipase-promoting agent, on gene induction of cytochrome P450s, carboxylesterases, and sulfotransferases in primary cultures of human hepatocytes. Nishimura, M., Imai, T., Morioka, Y., Kuribayashi, S., Kamataki, T., Naito, S. Drug Metab. Pharmacokinet. (2004) [Pubmed]
  17. Two glutathione S-transferase isoenzymes purified from Bulinus truncatus (Gastropoda: Planorbidae). Abdalla, A.M., El-Mogy, M., Farid, N.M., El-Sharabasy, M. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. (2006) [Pubmed]
  18. Induction of PNAd and N-acetylglucosamine 6-O-sulfotransferases 1 and 2 in mouse collagen-induced arthritis. Yang, J., Rosen, S.D., Bendele, P., Hemmerich, S. BMC Immunol. (2006) [Pubmed]
  19. A 96-well dot-blot assay for carbohydrate sulfotransferases. Verdugo, D.E., Bertozzi, C.R. Anal. Biochem. (2002) [Pubmed]
  20. Specificity of the lateral scapular slide test in asymptomatic competitive athletes. Koslow, P.A., Prosser, L.A., Strony, G.A., Suchecki, S.L., Mattingly, G.E. The Journal of orthopaedic and sports physical therapy. (2003) [Pubmed]
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