High impact information on Slc22a1

• The liver plays a major role in biotransformation and elimination of various therapeutic agents and xenobiotics, many of which are organic cations and substrates of the organic cation transporter 1 (Oct1, Slc22a1) [1].
• Nuclear extracts from PC12 cells contained a protein (or proteins) specifically binding the octamer sequence, and supershift experiments with anti-Oct-1 indicated involvement of this ubiquitously expressed transcription factor in the complex [2].
• An octamer 1 (Oct-1) binding site, located between positions -166 and -159 was shown by deletion analysis and site-directed mutation to be critical for promoter activity [2].
• Roct1, a rat polyspecific transporter gene for the excretion of cationic drugs, maps to chromosome 1q11-12 [3].
• Heat shock treatment suppresses angiotensin II-induced SP-1 and AP-1 and stimulates Oct-1 DNA-binding activity in heart [4].

Biological context of Slc22a1

• Systolic blood pressure was measured by tail-cuff plethysmography, expression of heat shock proteins was monitored by Western analysis and DNA-binding activities of SP-1, AP-1 and Oct-1 were determined by electrophoretic mobility shift assay [4].

Regulatory relationships of Slc22a1

• In addition, HS increased Oct-1 activity that was suppressed by Ang II infusion [4].

Other interactions of Slc22a1

• In this study we examined pro-inflammatory transcription factors SP-1, AP-1 and an anti-inflammatory cytokine transcriptional repressor, Oct-1, DNA-binding activities after chronic Ang II infusion and the effect of HS treatment on these pathways in heart [4].

References