High impact information on Tagln

• Calponin and SM22 alpha are major components of differentiated smooth muscle and potential regulators of actin cytoskeleton interactions [1].
• Vascular smooth muscle cell (VSMC) differentiation and phenotypic modulation is characterized by changes in mRNA expression for smooth muscle (SM) marker contractile proteins such as alpha-SM actin and SM22 alpha [2].
• Sm22 is predominantly associated with the actin cytoskeleton [3].
• Cloning and analysis of the promoter region of the rat SM22 alpha gene [4].
• The residual promoter activity in the smallest fragment of the SM22 alpha promoter tested suggested that, unlike the smooth-muscle alpha-actin promoter, transcription from the SM22 alpha promoter can occur in smooth-muscle cells in the absence of factors binding to CC(A/Trich)6GG (CArG box) or CANNTG (E box) motifs [4].

Biological context of Tagln

• We have used degenerate oligonucleotides, derived from the amino acid sequence of transgelin peptides [Shapland et al., 1993: J. Cell Biol. 121:1065-1073], to isolate and sequence overlapping cDNA clones encoding this actin gelling protein [5].
• Gene cloning and nucleotide sequence of SM22 alpha from the chicken gizzard smooth muscle [6].
• During postnatal development (3 days to 12 weeks), the expression of NOV: was correlated with markers of the differentiated smooth muscle cell phenotype (smooth muscle myosin heavy chain and SM22 alpha) [7].
• Transient reporter gene assays demonstrate that a 576-bp fragment, including the ID, contains a regulatory element which may contribute to the SMC-specific expression of SM22 alpha [8].
• The 5' untranslated leader sequence of the rat SM22 alpha gene was found to contain two introns of 3.6 and 2.9 kb [8].

Anatomical context of Tagln

• Regulation of SM22 alpha expression by arginine vasopressin and PDGF-BB in vascular smooth muscle cells [9].
• Transgelin is expressed as a single message that is regulated at the level of transcription in SV40 transformed 3T3 cells [5].
• Primers with 5' restriction enzyme sites directed against the N and C terminal amino acids present in these clones were then used to amplify and clone the entire transgelin coding region from reverse transcribed rat small intestine cDNA (RT-PCR) [5].
• A full length of cDNA encoding SM22 alpha from chicken gizzard smooth muscle was cloned and sequenced [6].

References