The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

3T3 Cells

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of 3T3 Cells

  • The EGF-RTA conjugate was toxic to 3T3 cells at concentrations (10(-9)--10(-11) M) similar to those at which EGF exerts its biological activity and within an order of magnitude of the toxicity of ricin [1].
  • The SV-40-transformed Balb/c 3T3 line (SVT2) contains four times less vinculin than the parental 3T3 cells, and the rat adenocarcinoma BSp73ASML has no detectable vinculin [2].
  • Mouse fibroblasts transformed by simian virus 40 (SV3T3 cells) are characterized by cyclic AMP and cyclic GMP levels, respectively, about half and twice those found in growing untransformed 3T3 cells [3].
  • A second phosphotyrosine-containing protein has been detected in both Rous sarcoma virus and Abelson murine leukemia virus-transformed 3T3 cells, but was absent from normal 3T3 cells and 3T3 cells transformed by various other viruses [4].
  • Although the amount of labeled p53 was increased in simian virus 40-transformed and methylcholanthrene-induced mouse sarcoma cells, the amount of p53 labeled during a 3-h pulse in Moloney virus- and Rous sarcoma virus-transformed cells and untransformed 3T3 cells was similar [5].

High impact information on 3T3 Cells


Chemical compound and disease context of 3T3 Cells


Biological context of 3T3 Cells

  • Synergistic stimulation of S6 ribosomal protein phosphorylation and DNA synthesis by epidermal growth factor and insulin in quiescent 3T3 cells [15].
  • Similarly, after treatment of 3T3 cells with the tumor-promoter phorbol myristate acetate, which induces a transformation-like phenotype, the cells no longer respond to PDGF as a chemoattractant but retain their migratory response to fibronectin [16].
  • Increased cAMP levels promoted by PDGF acting through stimulation of E-type prostaglandin synthesis may contribute to signal the initiation of cell proliferation in 3T3 cells [17].
  • Inhibitors of protein synthesis block the progression of quiescent 3T3 cells through G1 into S phase; however these drugs do not block the induction of KC and JE by PDGF [18].
  • When added to cultures under hitherto optimum conditions for epidermal cell growth [in the presence of supporting 3T3 cells and epidermal growth factor (EGF)], most of the agents exert an effect of considerable magnitude, the toxin being the most potent [19].

Anatomical context of 3T3 Cells


Associations of 3T3 Cells with chemical compounds

  • SSV-transformed cells have reduced numbers of high-affinity 125I-PDGF receptors; PDGF/p28v-sis receptor was purified from SSV-NIH 3T3 cells and retained active protein tyrosine kinase activity stimulated by PDGF [25].
  • Cyclic AMP-mediated control of lipogenic enzyme synthesis during adipose differentiation of 3T3 cells [26].
  • A transformed BHK cell lacking hypoxanthine guanosine phosphoribosyl tranferase, which was used an an indicator, was found to incorporate 3H-hypoxanthine when in prolonged (20 hr) or brief (1 hr) contact with donor 3T3 cells, whether the latter were in a quiescent layer of in the region of stimulation at the edge of a wound [27].
  • Interruption of the adipose conversion of 3T3 cells by biotin deficiency: differentiation without triglyceride accumulation [28].
  • In contrast, the neurohypophyseal hormone vasopressin, which is mitogenic for 3T3 cells, fails to synergize with TPA to stimulate DNA synthesis, ornithine decarboxylase activity or 2-deoxyglucose uptake [29].

Gene context of 3T3 Cells

  • Here we report that Rb can repress c-fos expression and AP-1 transcriptional activity in both serum-induced and cycling 3T3 cells [30].
  • Furthermore, Shh and downstream Shh signal transducers specifically induce ES enhancer/luciferase reporters in Shh-responsive 3T3 cells [31].
  • Surprisingly, although B7h is expressed in unstimulated B cells, its expression is induced in both 3T3 cells and embryonic fibroblasts treated with TNFalpha, and it is upregulated in nonlymphoid tissues of mice treated with LPS, a potent activator of TNFalpha [32].
  • Expression of a c-myc promoter driven CAT gene, transfected into quiescent 3T3 cells, is stimulated by serum addition whereas an identical gene containing mutations in the E2F binding sites is not responsive [33].
  • Contrary to c-jun and junB transcription, which are strongly stimulated by serum or TPA treatment of quiescent 3T3 cells, junD transcription is not significantly stimulated in these conditions [34].

Analytical, diagnostic and therapeutic context of 3T3 Cells


  1. Epidermal growth factor-toxin A chain conjugates: EGF-ricin A is a potent toxin while EGF-diphtheria fragment A is nontoxic. Cawley, D.B., Herschman, H.R., Gilliland, D.G., Collier, R.J. Cell (1980) [Pubmed]
  2. Suppression of tumorigenicity in transformed cells after transfection with vinculin cDNA. Rodríguez Fernández, J.L., Geiger, B., Salomon, D., Sabanay, I., Zöller, M., Ben-Ze'ev, A. J. Cell Biol. (1992) [Pubmed]
  3. Cyclic AMP and cyclic GMP concentrations in serum- and density-restricted fibroblast cultures. Moens, W., Vokaer, A., Kram, R. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  4. Four different classes of retroviruses induce phosphorylation of tyrosines present in similar cellular proteins. Cooper, J.A., Hunter, T. Mol. Cell. Biol. (1981) [Pubmed]
  5. Energy requirement for degradation of tumor-associated protein p53. Gronostajski, R.M., Goldberg, A.L., Pardee, A.B. Mol. Cell. Biol. (1984) [Pubmed]
  6. Tsg101: a novel tumor susceptibility gene isolated by controlled homozygous functional knockout of allelic loci in mammalian cells. Li, L., Cohen, S.N. Cell (1996) [Pubmed]
  7. The growth arrest-specific gene, gas1, is involved in growth suppression. Del Sal, G., Ruaro, M.E., Philipson, L., Schneider, C. Cell (1992) [Pubmed]
  8. Morphoregulatory activities of NCAM and N-cadherin can be accounted for by G protein-dependent activation of L- and N-type neuronal Ca2+ channels. Doherty, P., Ashton, S.V., Moore, S.E., Walsh, F.S. Cell (1991) [Pubmed]
  9. Characterization of the autoantigen La as a nucleic acid-dependent ATPase/dATPase with melting properties. Bachmann, M., Pfeifer, K., Schröder, H.C., Müller, W.E. Cell (1990) [Pubmed]
  10. A chicken-yeast chimeric beta-tubulin protein is incorporated into mouse microtubules in vivo. Bond, J.F., Fridovich-Keil, J.L., Pillus, L., Mulligan, R.C., Solomon, F. Cell (1986) [Pubmed]
  11. Sodium-stimulated alpha-aminoisobutyric acid transport by membrane vesicles from simian virus-transformed mouse cells. Hamilton, R.T., Nilsen-Hamilton, M. Proc. Natl. Acad. Sci. U.S.A. (1976) [Pubmed]
  12. Characterization of pp85, a target of oncogenes and growth factor receptors. Cohen, B., Liu, Y.X., Druker, B., Roberts, T.M., Schaffhausen, B.S. Mol. Cell. Biol. (1990) [Pubmed]
  13. Oligodendrocytes and CNS myelin are nonpermissive substrates for neurite growth and fibroblast spreading in vitro. Schwab, M.E., Caroni, P. J. Neurosci. (1988) [Pubmed]
  14. Difference in transport of leucine in attached and suspended 3T3 cells. Otsuka, H., Moskowitz, M. J. Cell. Physiol. (1975) [Pubmed]
  15. Synergistic stimulation of S6 ribosomal protein phosphorylation and DNA synthesis by epidermal growth factor and insulin in quiescent 3T3 cells. Nilsen-Hamilton, M., Hamilton, R.T., Allen, W.R., Potter-Perigo, S. Cell (1982) [Pubmed]
  16. Alteration of the chemotactic response of NIH/3T3 cells to PDGF by growth factors, transformation, and tumor promoters. Grotendorst, G.R. Cell (1984) [Pubmed]
  17. Platelet-derived growth factor elicits cyclic AMP accumulation in Swiss 3T3 cells: role of prostaglandin production. Rozengurt, E., Stroobant, P., Waterfield, M.D., Deuel, T.F., Keehan, M. Cell (1983) [Pubmed]
  18. Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cochran, B.H., Reffel, A.C., Stiles, C.D. Cell (1983) [Pubmed]
  19. Cyclic AMP in relation to proliferation of the epidermal cell: a new view. Green, H. Cell (1978) [Pubmed]
  20. Radioimmunoassay for tubulin: a quantitative comparison of the tubulin content of different established tissue culture cells and tissues. Hiller, G., Weber, K. Cell (1978) [Pubmed]
  21. The identification of calmodulin-binding sites on mitochondria in cultured 3T3 cells. Pardue, R.L., Kaetzel, M.A., Hahn, S.H., Brinkley, B.R., Dedman, J.R. Cell (1981) [Pubmed]
  22. Phagokinetic tracks of 3T3 cells: parallels between the orientation of track segments and of cellular structures which contain actin or tubulin. Albrecht-Buehler, G. Cell (1977) [Pubmed]
  23. A significant part of macrophage-derived growth factor consists of at least two forms of PDGF. Shimokado, K., Raines, E.W., Madtes, D.K., Barrett, T.B., Benditt, E.P., Ross, R. Cell (1985) [Pubmed]
  24. Inhibition by retinoic acid of murine retrovirus-induced cellular transformation and tumor formation. Giese, N.A., Neary, K.E., Levine, N., Lindell, T.J., Duffy, J.J. J. Natl. Cancer Inst. (1985) [Pubmed]
  25. Transforming protein of simian sarcoma virus stimulates autocrine growth of SSV-transformed cells through PDGF cell-surface receptors. Huang, J.S., Huang, S.S., Deuel, T.F. Cell (1984) [Pubmed]
  26. Cyclic AMP-mediated control of lipogenic enzyme synthesis during adipose differentiation of 3T3 cells. Spiegelman, B.M., Green, H. Cell (1981) [Pubmed]
  27. The effects of topoinhibition and cytochalasin B on metabolic cooperation. Stoker, M. Cell (1975) [Pubmed]
  28. Interruption of the adipose conversion of 3T3 cells by biotin deficiency: differentiation without triglyceride accumulation. Kuri-Harcuch, W., Wise, L.S., Green, H. Cell (1978) [Pubmed]
  29. Phorbol esters and vasopressin stimulate DNA synthesis by a common mechanism. Dicker, P., Rozengurt, E. Nature (1980) [Pubmed]
  30. Negative regulation of human c-fos expression by the retinoblastoma gene product. Robbins, P.D., Horowitz, J.M., Mulligan, R.C. Nature (1990) [Pubmed]
  31. Myf5 is a direct target of long-range Shh signaling and Gli regulation for muscle specification. Gustafsson, M.K., Pan, H., Pinney, D.F., Liu, Y., Lewandowski, A., Epstein, D.J., Emerson, C.P. Genes Dev. (2002) [Pubmed]
  32. B7h, a novel costimulatory homolog of B7.1 and B7.2, is induced by TNFalpha. Swallow, M.M., Wallin, J.J., Sha, W.C. Immunity (1999) [Pubmed]
  33. A role for the adenovirus inducible E2F transcription factor in a proliferation dependent signal transduction pathway. Mudryj, M., Hiebert, S.W., Nevins, J.R. EMBO J. (1990) [Pubmed]
  34. Characterization of junD: a new member of the jun proto-oncogene family. Hirai, S.I., Ryseck, R.P., Mechta, F., Bravo, R., Yaniv, M. EMBO J. (1989) [Pubmed]
  35. Role of transiently altered sarcolemmal membrane permeability and basic fibroblast growth factor release in the hypertrophic response of adult rat ventricular myocytes to increased mechanical activity in vitro. Kaye, D., Pimental, D., Prasad, S., Mäki, T., Berger, H.J., McNeil, P.L., Smith, T.W., Kelly, R.A. J. Clin. Invest. (1996) [Pubmed]
  36. Endogenous lectins from cultured cells: subcellular localization of carbohydrate-binding protein 35 in 3T3 fibroblasts. Moutsatsos, I.K., Davis, J.M., Wang, J.L. J. Cell Biol. (1986) [Pubmed]
  37. Epidermal growth factor induces rapid centrosomal separation in HeLa and 3T3 cells. Sherline, P., Mascardo, R.N. J. Cell Biol. (1982) [Pubmed]
  38. Signal transduction pathways in the induction of 2',5'-oligoadenylate synthetase gene expression by interferon alpha/beta. Yan, C., Sehgal, P.B., Tamm, I. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  39. Inducible production of c-fos antisense RNA inhibits 3T3 cell proliferation. Holt, J.T., Gopal, T.V., Moulton, A.D., Nienhuis, A.W. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
WikiGenes - Universities