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Col2a1  -  collagen, type II, alpha 1

Rattus norvegicus

Synonyms: Alpha-1 type II collagen, CG2A1A, COLLII, Collagen alpha-1(II) chain
 
 
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High impact information on Col2a1

  • In COS-7 cells, PIAS proteins stimulated the SOX9-dependent transcriptional activity of a Col2a1 promoter-enhancer reporter [1].
  • The transcription factor deltaEF1 is inversely expressed with type II collagen mRNA and can repress Col2a1 promoter activity in transfected chondrocytes [2].
  • A deletion analysis suggested that a region between -100 and -307 of the rat Col2a1 gene was required for activity in differentiating chick limb bud mesenchymal cells; however, mutation of a conserved E2 box site in this region actually increased promoter activity [2].
  • The type II collagen gene (Col2a1) is expressed primarily in chondrocytes [3].
  • Here, we map a minimal enhancer and identify elements that determine cartilage-specific Col2a1 expression by analyzing the activity of a series of chimeric genes consisting of rat Col2a1 first intron deletion mutants ligated to the chloramphenicol acetyltransferase reporter gene [3].
 

Biological context of Col2a1

  • These results suggest that interaction of chondrocyte specific nuclear factors with multiple core elements from a small region within the first intron are important for cell-type specific Col2a1 enhancer activity [3].
  • Sequence analysis of this 100-bp Col2a1 enhancer revealed several sequence motifs similar to motifs present within the regulatory region of the link protein gene, another cartilage gene [3].
 

Anatomical context of Col2a1

 

Other interactions of Col2a1

  • Markers of chondrocyte differentiation [alkaline phosphatase (ALP) activity, and type II (Col2a1) and type X collagen (Col10a1) expression] were enhanced by overexpression of Ihh or its N-terminal domain (N-Ihh), effects mimicked by exogenous administration of recombinant N-terminal HH peptide [4].
  • Here we present evidence that the transcription factor deltaEF1 participates in the negative regulation of Col2a1 transcription [2].

References

  1. Interactions between PIAS proteins and SOX9 result in an increase in the cellular concentrations of SOX9. Hattori, T., Eberspaecher, H., Lu, J., Zhang, R., Nishida, T., Kahyo, T., Yasuda, H., de Crombrugghe, B. J. Biol. Chem. (2006) [Pubmed]
  2. The transcription factor deltaEF1 is inversely expressed with type II collagen mRNA and can repress Col2a1 promoter activity in transfected chondrocytes. Murray, D., Precht, P., Balakir, R., Horton, W.E. J. Biol. Chem. (2000) [Pubmed]
  3. Identification of a minimum enhancer sequence for the type II collagen gene reveals several core sequence motifs in common with the link protein gene. Krebsbach, P.H., Nakata, K., Bernier, S.M., Hatano, O., Miyashita, T., Rhodes, C.S., Yamada, Y. J. Biol. Chem. (1996) [Pubmed]
  4. Ihh enhances differentiation of CFK-2 chondrocytic cells and antagonizes PTHrP-mediated activation of PKA. Deckelbaum, R.A., Chan, G., Miao, D., Goltzman, D., Karaplis, A.C. J. Cell. Sci. (2002) [Pubmed]
 
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