High impact information on STAT5A

• Binding was abolished by those two point mutations and super-shift analyses showed that STAT5A factors are present in this complex [1].
• STAT5A encoding gene maps to chromosome 19 in cattle and goat and to chromosome 11 in sheep [2].
• Nucleotide sequence variation in the transcription factor STAT5A 5'-noncoding region in Bos taurus and Bos indicus cattle [3].
• Milk protein genes expression in cows' mammary epithelial cells is regulated mostly by the action of prolactin mediated through the STAT5A transcription factor [4].
• For Black-and-White cows with different STAT5A genotypes no significant associations between STAT5A genotypes and milk performance traits were found [4].

Biological context of STAT5A

• With reverse transcription-PCR (RT-PCR) and other means we showed that the STAT5A expression level in the liver was higher in cattle with AA than GG genotype [5].
• Using PCR-heteroduplex and sequencing we identified A/G substitution at position -488 in the promoter region of the bovine STAT5A gene [5].
• The STAT5A gene is a potential quantitative trait locus (QTL) and genetic marker of production traits in dairy cattle [4].
• New SSCP polymorphism within bovine STAT5A gene and its associations with milk performance traits in Black-and-White and Jersey cattle [4].

Other interactions of STAT5A

• STAT5A, previously known as MGF, transduces prolactin signals to the milk protein genes [6].

Analytical, diagnostic and therapeutic context of STAT5A

• Electrophoretic mobility shift assay (EMSA) showed that the A-->G transition at position -488 increased the STAT5A gene promoter binding capacity for liver nuclear proteins [possibly hepatic nuclear factor (HNF)-3] [5].
• Ten PCR fragments representing most important functional domains of STAT5A were screened for polymorphism [4].

References