High impact information on PAX6

• Here, we show that bovine inner cell masses from nuclear transfer and fertilized embryos, grown on feeders in serum-free medium, form polarized rosette structures expressing nestin, Pax6, Pax7, Sox1, and Otx2 and exhibiting interkinetic nuclear migration activity and cell junction distribution as in the developing neural tube [1].
• In primates, these cells are organized into rosettes expressing Pax6 and Sox1 and are responsive to inductive signals such as Sonic hedgehog (Shh) and retinoic acid [1].
• Alternative splicing of Pax6 in bovine eye and evolutionary conservation of intron sequences [2].
• PAX6 expression identifies progenitor cells for corneal keratocytes [3].
• Cytometry demonstrated PAX6 protein in 4% of freshly isolated stromal cells [3].

Biological context of PAX6

• Additional alternative splicing products were identified which may lead to "domain swapping" or truncation of Pax6 proteins [2].
• These results demonstrate the presence of a previously unrecognized population of PAX6-positive cells in adult corneal stroma that maintain the potential to assume a keratocyte phenotype even after extensive replication [3].
• Assignment of the PAX6 gene to bovine chromosome 15q25-->q27 by fluorescence in situ hybridization and confirmation by radiation hybrid mapping [4].

Anatomical context of PAX6

• PAX6 protein was detected in the cultured progenitor cells and a small number of stromal cells in intact tissue but was absent in cultured keratocytes and fibroblasts [3].

References