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Gene Review

UUKVsLgp1  -  RNA polymerase

Uukuniemi virus

 
 
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Disease relevance of UUKVsLgp1

 

High impact information on UUKVsLgp1

  • We established a T7 RNA polymerase-driven reverse genetics system to rescue infectious clones of RVFV MP-12 strain entirely from cDNA, the first for any phlebovirus [5].
  • The particles are able to infect new cells, and reporter gene expression can be monitored if the trans-acting viral proteins (RNA polymerase and nucleoprotein) are also expressed in these cells [6].
  • We established an RVFV T7 RNA polymerase-driven minigenome system in which T7 RNA polymerase from an expression plasmid drove expression of RNA transcripts for viral proteins and minigenome RNA transcripts carrying a reporter gene between both termini of the M RNA segment in 293T cells [7].
  • CAT activity was determined after cotransfection with viral expression plasmids encoding the RNA-dependent RNA polymerase (L) and the nucleoprotein (N) or, alternatively, after superinfection with UUK virus helper virus [8].
  • When such cells were either superinfected with Uukuniemi virus or cotransfected with expression plasmids encoding the L (RNA polymerase), N (nucleoprotein), and NSs (nonstructural protein) viral proteins, strong CAT activity or GFP expression was observed [3].
 

Biological context of UUKVsLgp1

  • This was achieved by using a recently developed RNA polymerase I (Pol I)-driven reverse genetics system (R. Flick and R. F. Pettersson, J. Virol. 75:1643-1655, 2001) [8].
  • The L segment is 6404 nucleotides long, containing a single open reading frame (ORF) in the viral complementary sense coding for a protein of 2095 amino acids that, as in the case of negative strand RNA viruses, could be part of the RNA polymerase of TOS virus [9].

References

  1. Targeting TFIIH to inhibit host cell transcription by Rift Valley Fever Virus. Dasgupta, A. Mol. Cell (2004) [Pubmed]
  2. Uukuniemi virus contains an RNA polymerase. Ranki, M., Pettersson, R.F. J. Virol. (1975) [Pubmed]
  3. Reverse genetics system for Uukuniemi virus (Bunyaviridae): RNA polymerase I-catalyzed expression of chimeric viral RNAs. Flick, R., Pettersson, R.F. J. Virol. (2001) [Pubmed]
  4. Functional analysis of the noncoding regions of the Uukuniemi virus (Bunyaviridae) RNA segments. Flick, K., Katz, A., Overby, A., Feldmann, H., Pettersson, R.F., Flick, R. J. Virol. (2004) [Pubmed]
  5. Rescue of infectious rift valley fever virus entirely from cDNA, analysis of virus lacking the NSs gene, and expression of a foreign gene. Ikegami, T., Won, S., Peters, C.J., Makino, S. J. Virol. (2006) [Pubmed]
  6. Generation and analysis of infectious virus-like particles of uukuniemi virus (bunyaviridae): a useful system for studying bunyaviral packaging and budding. Overby, A.K., Popov, V., Neve, E.P., Pettersson, R.F. J. Virol. (2006) [Pubmed]
  7. Rift valley fever virus nonstructural protein NSs promotes viral RNA replication and transcription in a minigenome system. Ikegami, T., Peters, C.J., Makino, S. J. Virol. (2005) [Pubmed]
  8. Mutational analysis of the Uukuniemi virus (Bunyaviridae family) promoter reveals two elements of functional importance. Flick, R., Elgh, F., Pettersson, R.F. J. Virol. (2002) [Pubmed]
  9. Toscana virus genomic L segment: molecular cloning, coding strategy and amino acid sequence in comparison with other negative strand RNA viruses. Accardi, L., Grò, M.C., Di Bonito, P., Giorgi, C. Virus Res. (1993) [Pubmed]
 
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