Disease relevance of Uukuniemi virus

• The three RNA species isolated from virions of Uukuniemi virus, a proposed member of the newly defined Bunyaviridae family, have been characterized by analysis of 32P-labeled ribonuclease T1 oligonucleotides separated on two-dimensional polyacrylamide gels [1].
• A retention signal necessary and sufficient for Golgi localization maps to the cytoplasmic tail of a Bunyaviridae (Uukuniemi virus) membrane glycoprotein [2].
• We describe here the development of a reverse genetics system for the phlebovirus Uukuniemi virus, a member of the Bunyaviridae family, by using RNA polymerase I (pol I)-mediated transcription [3].

High impact information on Uukuniemi virus

• When such cells were either superinfected with Uukuniemi virus or cotransfected with expression plasmids encoding the L (RNA polymerase), N (nucleoprotein), and NSs (nonstructural protein) viral proteins, strong CAT activity or GFP expression was observed [3].
• Taken together, the results provide strong evidence that Golgi retention of the heterodimeric G1-G2 spike protein complex of Uukuniemi virus is mediated by a short region in the cytoplasmic tail of the G1 glycoprotein [4].
• Processing and membrane topology of the spike proteins G1 and G2 of Uukuniemi virus [5].
• Taken together, these results show that processing of p10 of Uukuniemi virus occurs cotranslationally at only one site, i.e., downstream of the internal G2 signal sequence [5].
• Effect of monensin on the assembly of Uukuniemi virus in the Golgi complex [6].

Chemical compound and disease context of Uukuniemi virus

• The internal ribonucleoprotein (RNP) of Uukuniemi virus was released with Triton X-100 and analyzed on sucrose gradients [7].

Gene context of Uukuniemi virus

• Uukuniemi virus contains an RNA polymerase [8].
• Association of the nonstructural protein NSs of Uukuniemi virus with the 40S ribosomal subunit [9].
• The transport of the secretory proteins fibronectin (FN) and procollagen (PC) was studied in cells infected with the temperature-sensitive mutant ts 12 of Uukuniemi virus [10].

References