Disease relevance of mobA

• A gene product related to Tral is required for the mobilization of Bacteroides mobilizable transposons and plasmids [1].
• MobA had homology to other Bacteroides mobilization proteins and RepA shared homology to a replication protein from Zymomonas mobilis plasmid pZM2 [2].
• Mobilization frequency was reduced 100- to 1,000-fold if the recipient was Escherichia coli but was not affected to the same extent if the recipient was an isogenic Bacteroides strain [3].

High impact information on mobA

• Phylogenetic analysis of MobA and several other mobilization proteins from commensal gastro-intestinal tract organisms suggested that they formed a new subgroup of the Tral superfamily [1].
• Conjugal transfer of the 5-nitroimidazole resistance plasmid pIP417 from Bacteroides vulgatus BV-17: characterization and nucleotide sequence analysis of the mobilization region [4].
• The two identified mobilization genes (mobA and mobB) are organized in one operon and were both required for efficient transfer [4].
• The NBU1 mobilization region contained a single large (1.4-kbp) open reading frame (ORF1), which was designated mob [5].
• Although functions on the Tcr elements were required for the excision-circularization and mobilization of NBU1, no Tcr element functions were required for integration into the recipient chromosome [6].

Biological context of mobA

• There were two open reading frames greater than 50 codons and these were designated mobA and repA [2].
• There was a marked difference in the G+C content and codon usage for the two regions; repA had 33% G+C and mobA was 44% G+C [2].
• The nucleotide sequence of the 2.1-kb DNA mobilization region was determined [4].
• The deduced amino acid sequence of the mob product had no significant similarity to those of mobilization proteins of well-characterized Escherichia coli group plasmids such as RK2 or of either of the two mobilization proteins of Bacteroides plasmid pBFTM10 [5].

Associations of mobA with chemical compounds

• Southern blotting indicated that the mobilization region of plasmid pIP417 is closely related to that of both the erythromycin resistance plasmid pBFTM1O and the 5-Ni resistance plasmid pIP419 but not to that of the 5-Ni resistance plasmid pIP421 [4].

Analytical, diagnostic and therapeutic context of mobA

• Using PCR and mobilization assays it was established that excision was stimulated by TET in the presence of CTn341 [7].
• These constructs (pKBR23-1 and pKBR23-2) were transferred into Bacteriodes distasonis, Bacteroides thetaiotaomicron, Bacteroides uniformis and into P. ruminicola NCFB 2202 either by conjugal mobilization or by electroporation [8].

References