Disease relevance of F gene

• A cDNA clone encoding the entire F gene of the live attenuated mumps virus (MpsV) strain, Urabe Am9, has been isolated and sequenced [1].
• The F gene of PIV-2 is most closely related to those of simian virus 5 (SV5) and mumps virus (MuV) [2].
• Comparison of the HPIV3 F protein sequence with the F gene sequences of two other paramyxoviruses, Sendai virus and simian virus 5, indicated a very close evolutionary relationship between HPIV3 and Sendai virus [3].
• cDNA clones representing the fusion (F) gene of human parainfluenza virus type 2 (PIV-2) were isolated from cDNA libraries constructed from virus-specific mRNA and genomic RNA, and the complete nucleotide sequence of the F gene was determined [2].

High impact information on F gene

• Syncytium formation was induced when COS7 cells were transfected with both recombinant plasmid DNAs together, but not with the recombinant plasmid only carrying the F gene [4].
• It was striking that there was no apparent consensus gene end sequence between the M and the F genes and that the M gene was transcribed exclusively as a dicistron with the F gene [5].
• It was found, in addition, that one of these strains (Enders strain) contained a point mutation in putative polyadenylation signal for the F gene mRNA (TTTAGAAAAAAA to TTTAGAAGAAAA) [6].
• The F gene is 1854 nucleotides long and encodes one long open reading frame of 551 amino acids [2].
• Then, the F gene of genotypes G, H, and K strains were analyzed together with previously reported strains in this study [7].

Biological context of F gene

• The HPIV3 F gene contains a single long open reading frame coding for 539 amino acids [3].
• The complete nucleotide sequence of the F gene was determined [8].
• The F gene of the SBL strain was compared to that of the RW strain [Waxham et al. (1987) Virology 159, 381-388] [9].
• Two resistant mutants were raised and shown to have single point mutations in the F-gene (S398L and D486N) [10].
• Phylogenetic analysis of the ophidian paramyxoviruses resulted in three clusters for the L-gene sequence and corresponding clusters for the F-gene sequence, indicating no species specificity [11].

Analytical, diagnostic and therapeutic context of F gene

• A PCR assay coupled with the sequencing of the 5' end of the F gene seems to be a convenient method for characterizing mumps virus strains [12].
• RT-PCR of the partial L-gene (566 nt) was successful for all 18 viruses; amplicons of the partial F-gene (918 nt) could be obtained in 16 cases [11].

References