Disease relevance of Tbh

• Fertility of the mutant females is also restored when TBH is expressed, via the GAL4-UAS system, in cells of the CNS abdominal ganglion that express TBH and produce octopamine [1].

High impact information on Tbh

• T beta h-null flies are octopamine-less but survive to adulthood [2].
• The deduced amino acid sequence of the encoded protein exhibits 39% identity to the evolutionarily related mammalian dopamine beta-hydroxylase enzyme [2].
• These data demonstrate that the endogenous signal peptide is not required for the formation of the membranous dopamine beta-hydroxylase and further that the enzyme can be bound to membranes via a mechanism other than uncleaved signal sequence [3].
• Finally, I demonstrate that these TBH-expressing cells project to the periphery where they innervate the ovaries and the oviducts of the reproductive system [1].

Analytical, diagnostic and therapeutic context of Tbh

• Here we report the molecular cloning of the Drosophila melanogaster gene, which encodes tyramine beta-hydroxylase (TBH), the enzyme that catalyzes the last step in octopamine biosynthesis [2].
• The inducible expression of active dopamine beta-hydroxylase in these cells has been verified by Western blotting and enzyme activity assays [3].

References