Disease relevance of SA

• The DSA protein has been cloned and expressed in a baculovirus system, and polyclonal antibodies were generated against the recombinant molecule [1].

High impact information on SA

• Drosophila nipped-B protein supports sister chromatid cohesion and opposes the stromalin/Scc3 cohesion factor to facilitate long-range activation of the cut gene [2].
• Serial transovarial passage of SA virus in Ae. albopictus did not alter its plaque morphology, infectivity for mosquitoes, or pathogenicity for vertebrates [3].
• The behavior of SA virus in Ae. albopictus was similar to that of sigma virus in Drosophila melanogaster, suggesting that some females in the transovarial transmission-efficient line had developed a chronic infection of their germinal cells (oogonia) [3].
• This cDNA fragment was used as a radiolabeled probe for screening a cDNA library from Drosophila embryos, and we have cloned a full-length cDNA for the SA homolog from an insect [1].
• The in situ hybridization experiments indicate that the DSA messenger is expressed mainly in neurogenic territories in the embryonic development of Drosophila [1].

Analytical, diagnostic and therapeutic context of SA

• Taking into account the identical domain present in all the SA family members characterized to date, we have carried out polymerase chain reaction (PCR) using degenerate oligonucleotides from the 5' and 3' ends of one of those regions of the molecule and cDNA from D. melanogaster embryos [1].
• Molecular cloning and expression of stromalin protein from Drosophila melanogaster: homologous to mammalian stromalin family of nuclear proteins [1].

References