High impact information on Aats-ala

• Second, we find that the specific sequences bound to the yeast nuclear scaffold are the putative origins of replication (ARS elements) and a chromosomal centromere, CENIII [1].
• The data establish a close relationship between the sequences involved in ARS activity and scaffold binding: ARS elements that can bind the nuclear scaffold in vitro promote more efficient plasmid replication in vivo, but scaffold association is not a strict prerequisite for ARS function [2].
• Drosophila scaffold-attached regions bind nuclear scaffolds and can function as ARS elements in both budding and fission yeasts [3].
• A comparison of the enhancer sequence with the sequences of 14 different ARS elements failed to reveal significant homology areas [4].
• An ARS element from Drosophila melanogaster telomeres contains the yeast ARS core and bent replication enhancer [4].

Biological context of Aats-ala

• Yeast rDNA was previously shown to contain a sequence capable of autonomous replication (ARS) (Szostak, J.W. and Wu, R (1979), Plasmid 2, 536-554) [5].
• This ARS, which may correspond to a chromosomal origin of replication, was located on a fragment of 570 basepairs within NTS2 [5].
• Other sequences found in the 37639 bp fragment are one delta and one solo-sigma element, the tRNA-Arg3 gene, the small nuclear RNA gene SNR6 and three ARS consensus sequences [6].
• Two DNA segments exhibiting ARS (autonomously replicating sequence) activity in the dimorphic yeast Yarrowia lipolytica were cloned from its chromosome on an integrative LEU2 plasmid [7].

Anatomical context of Aats-ala

• Chromosomal ARS and CEN elements bind specifically to the yeast nuclear scaffold [1].

References