Disease relevance of IGLC3

• For CML, the breakpoint is distal to the IGLC genes, whereas for Burkitt lymphoma it is proximal [1].
• We have performed in situ hybridization of a probe for the lambda IGLC constant region to metaphase spreads from two DiGeorge syndrome (DGS)-related chromosomal rearrangements with breakpoints in 22q11 [2].
• Two of the three meningiomas maintained constitutional heterozygosity at the IGLC locus and another one showed no loss of heterozygosity at IGLC or D22S9 [3].

High impact information on IGLC3

• Recently, we demonstrated that Southern blot analysis with the IGLC3 probe in combined EcoRI/HindIII digests allows detection of approximately 95% of all clonal Ig(lambda) gene rearrangements in B cell malignancies [4].
• Our extensive Southern blot analyses with the IGLC3 probe in EcoRI and/or HindIII digests revealed that combined EcoRI/HindIII digestion detected Ig lambda gene rearrangements in 95% of the 75 patients and 94% of the 98 rearranged alleles [5].
• We employed exon trapping and large-scale genomic sequence analysis of two bacterial artificial chromosome clones to isolate genes from the region between the IGLC and BCR in chromosome 22q11 [6].
• Independent segregation analysis of the related owl monkey DNA sequences for these two genes demonstrated that both the SIS locus and the IGLC locus are localized on owl monkey chromosome 3 of karyotype VI, chromosome 3 of karyotype V, and chromosome 6 of karyotype IV [7].
• Two cloned human DNA sequences specific for the SIS and IGLC genes were hybridized with DNAs from panels of rodent-owl monkey somatic cell hybrids [7].

Biological context of IGLC3

• The study provides direct evidence for regional assignment of the IGLC gene cluster to 22q11 [1].
• Synteny of SIS and IGLC in owl monkeys. Evidence for karyotype evolution [7].
• Then, we compared constitutional and tumor genotypes from 14 cases of sporadic human meningiomas, using four polymorphic DNA probes on chromosome 22 (SIS, D22S1, D22S9, IGLC) [3].

References