High impact information on 1.28

• We also tested the ability of the chimeric proteins to regulate transcription units that are normal targets of Deformed and Ultrabithorax [1].
• High-affinity binding sites for the Deformed protein are required for the function of an autoregulatory enhancer of the Deformed gene [2].
• Consistent with this hypothesis, we have discovered DEAF-1 binding sites in other Deformed response elements [3].
• Specific activation requires a second region in the 120 bp element, which presumably contains one or more binding sites for Deformed cofactors [3].
• A 120 bp homeotic response element that is regulated specifically by Deformed in Drosophila embryos contains a single binding site for Deformed protein [3].

Biological context of 1.28

• The 1.28 DRE contains in vitro binding sites for Deformed and DEAF-1 [4].
• Comparisons of the 1.28 DRE with other known Deformed-responsive enhancers indicate that there are multiple ways to construct Deformed Response Elements [4].
• We identified a 664-bp Deformed Response Element (1.28 DRE) that directs maxillary-specific expression of a reporter gene in transgenic embryos [4].
• But the polyacrylamide gel method uncovered two multiband homozygotes, alpha(d).77 and beta(d) 1.28 [5].
• Here we identify and characterize one such target gene, a gene activated by the product of the homeotic gene Deformed [6].

Associations of 1.28 with chemical compounds

• As we predicted, methoprene phenocopies a subset of previously described BRC defects; it also phenocopies Deformed and produces abnormalities not associated with known mutations [7].

Physical interactions of 1.28

• The Deformed binding sites do not have the consensus sequence for cooperative binding with the cofactor Extradenticle, and we do not detect cooperative binding to these sites, though we cannot rule out an independent role for Extradenticle [4].

Other interactions of 1.28

• DEAF-1, a novel protein that binds an essential region in a Deformed response element [3].
• 01, and 0.62 +/- 0.06 microM, respectively. cGMP activation was cooperative with a Hill coefficient (nH) of 1.28 +/- 0.10, whereas activation by cAMP was not cooperative [8].
• We propose that cnc functions in combination with the homeotic gene Deformed to specify mandibular development [9].
• We undertook a deficiency screen in Drosophila melanogaster in an attempt to identify loci potentially encoding cofactors for the protein encoded by the HOM-C gene Deformed (Dfd) [10].
• One of these genes, extradenticle, encodes a homeodomain transcription factor that heterodimerizes with Deformed and other homeotic Hox proteins [11].

Analytical, diagnostic and therapeutic context of 1.28

• Here we describe a detailed functional dissection of a small element that is specifically regulated by the Deformed homeotic protein [12].

References