Disease relevance of mu2

• A yeast artificial chromosome spanning this region has been subcloned into lambda phage, and the positions of deficiency breakpoints on either side of the mu2 gene have been identified within the subclones [1].

High impact information on mu2

• Terminal deficiencies at the tip of the X chromosome can be induced at a high frequency (0.2-0.3%) by irradiating Drosophila females carrying a homozygous mutator (mu-2) with low doses of X-rays [2].
• We have mapped and cloned the mu2 gene and rescued the mu2 phenotype by germ-line transformation with genomic DNA fragments containing the mu2 gene [3].
• Our data show that mu2 represents a novel component in the processing of double strand breaks (DSBs) in female meiosis. mu2 does not code for a DNA repair enzyme because mu2 mutants are not hypersensitive to DSB-inducing agents [3].
• Our genetic and molecular data are consistent with the model that mu2 encodes a structural component of the oocyte nucleus [3].
• The 45-4 line carries a white transgene inserted at 16 kb from the terminus and is homozygous for the mu-2 mutation, a gene involved in the repair of double-strand DNA breaks [4].

Biological context of mu2

• We propose that mu2 affects chromosomal structure during oogenesis, thereby modulating DNA repair [5].

Anatomical context of mu2

• Germ-line effects of a mutator, mu2, in Drosophila melanogaster [5].

Analytical, diagnostic and therapeutic context of mu2

• Molecular cloning and tissue-specific expression of the mutator2 gene (mu2) in Drosophila melanogaster [3].

References