High impact information on PAX7

• Regionalized cadherin-7 expression by radial glia is regulated by Shh and Pax7 during chicken spinal cord development [1].
• In cultures progressing from proliferation to differentiation, the expression of Pax7 in MyoD+ cells declined as the cells began expressing myogenin, suggesting Pax7 as an early marker for proliferating myoblasts [2].
• Pattern of Pax7 expression during myogenesis in the posthatch chicken establishes a model for satellite cell differentiation and renewal [2].
• At all time points, some Pax7+ cells were negative for MyoD, resembling the reserve cell phenotype [2].
• The paired-box transcription factor Pax7 plays a critical role in the specification of satellite cells in mouse skeletal muscle [2].

Biological context of PAX7

• The role for Pax7 in establishing tectal polarity and map refinement was authenticated by gene expression studies in vivo and in vitro [3].
• This increased cell number was accompanied by higher expression levels of Pax7 and myogenin proteins on posthatch days 1 and 3, respectively [4].

Anatomical context of PAX7

• The distinctions in Pax7 and myogenin expression patterns, both in culture and in vivo, indicate that while some of the myoblasts differentiate and fuse into myofibers during early stages of posthatch growth, others retain their reserve cell capacity [2].
• Throughout development (stages E2-E12 were examined) a rostral(low)-caudal(high) and dorsal(high)-ventral(low) Pax7 expression gradient was detected immunohistochemically in the chick optic tectum, indicating a role for Pax7 in establishing tectal polarity [3].
• Specification of the neural crest occurs during gastrulation and requires Pax7 [5].
• Whilst initial Pax7-specification of the mesencephalon is well-established, a role in regulating polarity within the maturing mouse superior colliculus is yet to be defined, although already detailed for the chick tectum [6].
• Pax7 is pivotal in specifying the superior colliculus/tectum, an important centre for integration of visuomotor responses and a target for Pax6 ( + ) retinal ganglion cell axons during retinocollicular mapping [6].

Other interactions of PAX7

• In particular, PAX6 and PAX7 are expressed in restricted regions in the CNS during early stages [7].

Analytical, diagnostic and therapeutic context of PAX7

• To study the regulation of the Cad7 gene, we ectopically expressed two known dorsoventral patterning genes, Shh and Pax7, in the caudal neural tube and in two brain regions at different stages of development by in vivo electroporation [1].

References