Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene Review:

GATA-6 - transcription factor GATA-6

Sus scrofa

Gillio-Meina, C. et al., Markmann, A. et al.

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High impact information on GATA-6

Immunoblot analyses showed two predominant proteins with molecular masses of approximately 53 and 55 kDa for GATA-4 and one 55-kDa protein for GATA-6 [1].
Cotransfection of primary cultures of luteinizing granulosa cells with GATA-4 or GATA-6 expression vectors increased the activity of the porcine steroidogenic acute regulatory protein gene promoter significantly but did not significantly activate the inhibin alpha gene promoter [1].
Immunohistochemical studies revealed GATA-4 and GATA-6 nuclear staining in granulosa cells of healthy primordial and primary antral follicles and antral follicle of various sizes [1].
Immunoreactivity for GATA-4 and GATA-6 was present in most blood vessels [1].
In electrophoretic mobility shift assays, nuclear protein extracts isolated from granulosa cells and CL exhibited both GATA-4 and GATA-6 binding to a GATA consensus oligonucleotide, with GATA-4 the predominant binding protein [1].

Biological context of GATA-6

The expression and localization of GATA-4 and GATA-6 mRNAs and proteins were assessed in porcine ovaries at different stages of the estrous cycle [1].
Reverse transcription polymerase chain reaction and Western blot analyses revealed that GATA-4 and GATA-6 transcripts and proteins were strongly expressed in granulosa cells isolated from antral follicles, intact antral follicles, corpora hemorrhagica (CH), and midluteal phase corpora lutea (CL) [1].
The transcription factors Gax and GATA-6 are considered negative, and Oct-1 positive regulators of the synthetic phenotype [2].
The serum-mediated down-regulation of Gax was more intense than that of GATA-6, and stronger in explant-derived than in primary cells [2].

Anatomical context of GATA-6

Since the phenotype transition can be induced by culturing the cells with serum, we followed the expression of Gax, GATA-6 and Oct-1, integrins and matrix genes in quiescent porcine vascular smooth muscle cells after serum application [2].

References

GATA-4 and GATA-6 transcription factors: expression, immunohistochemical localization, and possible function in the porcine ovary. Gillio-Meina, C., Hui, Y.Y., LaVoie, H.A. Biol. Reprod. (2003) [Pubmed]
Expression of transcription factors and matrix genes in response to serum stimulus in vascular smooth muscle cells. Markmann, A., Rauterberg, J., Vischer, P., Robenek, H., Echtermeyer, F., Will, H., Seidler, D.G., Young, M.F., Kresse, H. Eur. J. Cell Biol. (2003) [Pubmed]

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