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Gene Review:

hba1 - hemoglobin, alpha 1

Xenopus laevis

Stalder, J., Banville, D. et al., Ramseyer, L.T. et al., Kay, R.M. et al., Koibuchi, N. et al., et al.

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High impact information on LOC397749

Here, we report that depletion of zygotic xNAP1L in embryos produces no obvious morphologic phenotype, but ablates alpha-globin mRNA expression in the VBIs [1].
RNA in situ analysis revealed a significant reduction (or absence) of stem cell leukemia (SCL), alpha-globin, and GATA-1 expression, but not GATA-2 expression [2].
We also demonstrate that the first generations of erythrocytes, already express the predominating larval-specific alpha-globin mRNAs [3].
Nucleotide sequence of a larval alpha globin gene from Xenopus tropicalis [4].
Insertion of 1.5 kb of the 5' flanking region of the adult alpha-globin gene of X. laevis in front of the CAT structural gene promotes synthesis of CAT in transiently transfected X. laevis kidney cells [5].

Biological context of LOC397749

Sufficient DNA sequence information has been determined from each cDNA clone to demonstrate that pXG8D2 contains a beta-globin sequence and pXG6C1 contains an alpha-globin sequence [6].
A fourth tadpole alpha-globin mRNA sequence, for which we do not have a cDNA clone, accumulates co-ordinately with the three major mRNA sequences but to a much lower concentration [7].
The pattern of expression of the Xenopus laevis tadpole alpha-globin genes and the amino acid sequence of the three major tadpole alpha-globin polypeptides [7].
Erythropoiesis in these explants is demonstrated by whole mount in situ hybridization using an embryonic alpha-globin probe [8].

Anatomical context of LOC397749

The stimulation is specific for the alpha-globin promoter and is obtained after fusion with erythroid cells but not with hepatocytes or kidney cells [5].
The synthesis of endogenous proteins in oocytes and that of alpha-globin in the in vitro system were not affected by anti-beta-globin oligonucleotides [9].

Associations of LOC397749 with chemical compounds

Dot blots and Northern blots of total RNA from the heterokaryons contained material that reacted with an adult Rana alpha-globin probe; newly synthesized adult Rana hemoglobin tetramers were detected with native polyacrylamide gel electrophoresis [10].

References

Zygotic nucleosome assembly protein-like 1 has a specific, non-cell autonomous role in hematopoiesis. Abu-Daya, A., Steer, W.M., Trollope, A.F., Friedeberg, C.E., Patient, R.K., Thorne, A.W., Guille, M.J. Blood (2005) [Pubmed]
Essential role of HGF (hepatocyte growth factor) in blood formation in Xenopus. Koibuchi, N., Kaneda, Y., Taniyama, Y., Matsumoto, K., Nakamura, T., Ogihara, T., Morishita, R. Blood (2004) [Pubmed]
The switch from larval to adult globin gene expression in Xenopus laevis is mediated by erythroid cells from distinct compartments. Weber, R., Blum, B., Müller, P.R. Development (1991) [Pubmed]
Nucleotide sequence of a larval alpha globin gene from Xenopus tropicalis. Knöchel, W., Pfanne, K., Beck, J., Meyerhof, W. Nucleic Acids Res. (1988) [Pubmed]
Erythroid specific activation of the Xenopus laevis adult alpha-globin promoter in transient heterokaryons. Stalder, J. Nucleic Acids Res. (1988) [Pubmed]
Molecular cloning of cDNA sequences coding for the major alpha- and beta-globin polypeptides of adult Xenopus laevis. Kay, R.M., Harris, R., Patient, R.K., Williams, J.G. Nucleic Acids Res. (1980) [Pubmed]
The pattern of expression of the Xenopus laevis tadpole alpha-globin genes and the amino acid sequence of the three major tadpole alpha-globin polypeptides. Banville, D., Williams, J.G. Nucleic Acids Res. (1985) [Pubmed]
Induction of erythropoiesis in the amphibian embryo. Knöchel, W. Ann. N. Y. Acad. Sci. (1994) [Pubmed]
Anti-messenger oligodeoxynucleotides: specific inhibition of rabbit beta-globin synthesis in wheat germ extracts and Xenopus oocytes. Cazenave, C., Loreau, N., Toulmé, J.J., Hélène, C. Biochimie (1986) [Pubmed]
Intracellular signals for developmental hemoglobin switching. Ramseyer, L.T., Barker-Harrel, J., Smith, D.J., McBride, K.A., Jarman, R.N., Broyles, R.H. Dev. Biol. (1989) [Pubmed]

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