Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

slc1a3 - solute carrier family 1 (glial high...

Xenopus laevis

Synonyms: ea6, eaat1, glast, glast-1, glast1

Larsson, H.P. et al., Do, S.H. et al., Fairman, W.A. et al., Tzingounis, A.V. et al., Yun, J.Y. et al., et al.

Larsson, Tzingounis, Koch, Kavanaugh,

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on MGC68789

In Xenopus laevis oocytes expressing the excitatory amino acid transporter EAAT4, physiologically relevant concentrations of arachidonic acid increase the amplitude of the substrate-activated current by roughly twofold at -60 mV [1].
In the present study, we used voltage-clamp fluorometry to measure conformational changes in the neuronal excitatory amino acid transporter (EAAT) 3 glutamate transporter covalently labeled with a fluorescent reporter group [2].
The excitatory amino acid transporter EAAT4 is expressed predominantly in Purkinje neurons in the rat cerebellum (1-3), and it participates in postsynaptic reuptake of glutamate released at the climbing fiber synapse (4) [3].
Although chelerythrine did not change the anesthetic effects on the activity of EAAT3, staurosporine or calphostin C abolished the anesthetic-induced increase of EAAT3 activity [4].
Isoflurane (0.70 mm) significantly increased Vmax (maximum velocity) (3.6 +/- 0.4 to 5.1 +/- 0.4 microC; P < 0.05) but not Km (Michoelis-Menten Constant) (55.4 +/- 17.0 vs. 61.7 +/- 13.6 microm; P > 0.05) of EAAT3 for glutamate compared with control [4].

Associations of MGC68789 with chemical compounds

RESULTS: Ethanol enhanced EAAT3 activity in a concentration-dependent manner [5].
We investigated the effects of the intravenous anesthetics, thiopental, etomidate and ketamine, on the activity of one type of glutamate transporters, EAAT3 (excitatory amino acid transporter type 3) [6].

Other interactions of MGC68789

Pretreatment with wortmannin, a PI3 K inhibitor, also abolished the ethanol-enhanced EAAT3 activity [5].

References

Arachidonic acid elicits a substrate-gated proton current associated with the glutamate transporter EAAT4. Fairman, W.A., Sonders, M.S., Murdoch, G.H., Amara, S.G. Nat. Neurosci. (1998) [Pubmed]
Fluorometric measurements of conformational changes in glutamate transporters. Larsson, H.P., Tzingounis, A.V., Koch, H.P., Kavanaugh, M.P. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
Arachidonic acid activates a proton current in the rat glutamate transporter EAAT4. Tzingounis, A.V., Lin, C.L., Rothstein, J.D., Kavanaugh, M.P. J. Biol. Chem. (1998) [Pubmed]
Effects of volatile anesthetics on glutamate transporter, excitatory amino acid transporter type 3: the role of protein kinase C. Do, S.H., Kamatchi, G.L., Washington, J.M., Zuo, Z. Anesthesiology (2002) [Pubmed]
Effects of ethanol on the rat glutamate excitatory amino acid transporter type 3 expressed in Xenopus oocytes: role of protein kinase C and phosphatidylinositol 3-kinase. Kim, J.H., Lim, Y.J., Ro, Y.J., Min, S.W., Kim, C.S., Do, S.H., Kim, Y.L., Zuo, Z. Alcohol. Clin. Exp. Res. (2003) [Pubmed]
Effects of intravenous anesthetics on the activity of glutamate transporter EAAT3 expressed in Xenopus oocytes: evidence for protein kinase C involvement. Yun, J.Y., Kim, J.H., Kim, H.K., Lim, Y.J., Do, S.H., Zuo, Z. Eur. J. Pharmacol. (2006) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg