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MeSH Review


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Disease relevance of Fluorometry


High impact information on Fluorometry

  • Quantitation of hindered rotations of diphenylhexatriene in lipid bilayers by differential polarized phase fluorometry [6].
  • Transcriptional analysis, fluorometry, and flow cytometry revealed evidence of salicylic acid-mediated activation of the stress-response gene sigB [7].
  • Expression of mutant and wild-type receptors was assessed by Western analysis, and the effects of the mutation on extracellular calcium (Ca2+(o)) and gadolinium (Gd3+(o)) elicited increases in the cytosolic calcium concentration (Ca2+(i)) were examined in fura-2-loaded cells using dual wavelength fluorimetry [8].
  • To examine the hypothesis that glutathione S-transferases (GST) play an important role in the hepatocellular transport of hydrophobic organic anions, the kinetics of the spontaneous transfer of unconjugated bilirubin between membrane vesicles and rat liver glutathione S-transferase B (ligandin) was studied, using stopped-flow fluorometry [9].
  • In this study, we have determined NHE kinetics in cultured lymphoblasts from diabetic patients with and without nephropathy, with nondiabetic controls, using fluorometry with the pH indicator 2,7'-bis-(carboxyethyl)-5,6-carboxyfluorescein and estimation of NHE isoform 1 (NHE-1) density with specific polyclonal antibodies [10].

Chemical compound and disease context of Fluorometry


Biological context of Fluorometry


Anatomical context of Fluorometry

  • Studies of the effect of NO on [Ca2+]i and the rate of Mn2+ influx with fura-2 fluorometry in rabbit aortic smooth muscle cells in primary culture were designed to test how SERCA is involved in mediating the response to NO [17].
  • Using stopped-flow fluorimetry and a quenched-flow approach, we provide evidence for the involvement of cofilin in actin filament reorganization and SOCE in platelets [18].
  • To characterize the mechanisms underlying intracellular bilirubin transport, stopped-flow fluorometry was utilized to study the effects of membrane vesicle size and lipid composition on the kinetics of unconjugated bilirubin movement between model and native hepatocyte membranes [19].
  • Despite comparable proliferative responses, NOD CD4(+) T cells had significantly increased IFN-gamma intracellular/extracellular protein and mRNA responses compared with B6.G7 T cells as measured by intracellular cytokine analysis, time resolved fluorometry, and RNase protection assays [20].
  • The effects of the insulin-releasing sulfonylurea tolbutamide on the cytoplasmic Ca2+ concentration [( Ca2+]i) in individual pancreatic beta-cells or suspensions of beta-cells were analyzed using the probe fura-2 and dual-wavelength fluorometry [21].

Associations of Fluorometry with chemical compounds

  • Fluorometry with the dye, mag-fura-2, was used to characterize intracellular Mg2+ concentration ([Mg2+]i) in single cTAL cells [22].
  • The prostaglandin derivatives are detected directly on the microcolumn by laser fluorimetry, using a helium/cadmium laser (325 nm, 15 mW) as the excitation source together with a simple filter/photo-multiplier optical detection system [23].
  • The release did not depend on Ca2+ release from the intraterminal Ca2+ stores either because fura-2 fluorimetry showed extremely low Ca2+ elevation (approximately 30 nM) in response to nicotine (30 nM) [24].
  • The number of bound liposomes could be evaluated by fluorometry, and the internalization of liposomes was evaluated by the methotrexate-mediated inhibition of radio-labeled deoxyuridine incorporation [25].
  • Using front-surface fluorometry, we determined the effects of cocaine on force and cytosolic Ca2+ concentration ([Ca2+]i) in the rat aorta [26].

Gene context of Fluorometry

  • We have applied time-resolved fluorometry (TRF) to construct a DNA hybridization assay for the diagnosis of Leber hereditary optic neuroretinopathy (LHON) [27].
  • 5. The fraction of the total cation current through NMDA-R that was carried by Ca2+, measured using a combination of patch clamp and fluorometry in neurones loaded with a high concentration of the Ca2+ indicator fura-2, was found to be approximately 12% [28].
  • Binding of DDR2 complexes to collagen I coated on plastic plates was established by a microplate-based assay using Eu(3+)-labeled proteins and time-resolved fluorometry [29].
  • The dissociation constant for this conjugate was similar to that measured for [125I]IL8 ( approximately 2 nM) when measured by time-resolved fluorometry using CHO cell lines stably expressing CXCR1 or CXCR2 receptors [30].
  • Histamine (fluorimetry), LTC(4) (RIA) and IL-4 (ELISA) were all assayed using the same 4-h culture supernatants [31].

Analytical, diagnostic and therapeutic context of Fluorometry


  1. Exogenous thymidine is preferentially incorporated into human cytomegalovirus DNA in infected human fibroblasts. Morin, J., Johann, S., O'Hara, B., Gluzman, Y. J. Virol. (1996) [Pubmed]
  2. Effect of hemolysis on serum retinol as assessed by direct fluorometry. Marinovic, A.C., May, W.A., Sowell, A.L., Khan, L.K., Huff, D.L., Bowman, B.A. Am. J. Clin. Nutr. (1997) [Pubmed]
  3. Thermodynamics of active-site ligand binding to Escherichia coli glutamine synthetase. Ginsburg, A., Gorman, E.G., Neece, S.H., Blackburn, M.B. Biochemistry (1987) [Pubmed]
  4. Pyridine nucleotides of rabbit cornea with histotoxic anoxia: chemical analysis, non-invasive fluorometry and physiological correlates. Masters, B.R., Riley, M.V., Fischbarg, J., Chance, B. Exp. Eye Res. (1983) [Pubmed]
  5. Nature of [Ca2+]i transients during ventricular fibrillation and quinidine treatment in perfused rat hearts. Kojima, S., Wikman-Coffelt, J., Wu, S.T., Parmley, W.W. Am. J. Physiol. (1994) [Pubmed]
  6. Quantitation of hindered rotations of diphenylhexatriene in lipid bilayers by differential polarized phase fluorometry. Lakowicz, J.R., Prendergast, F.G. Science (1978) [Pubmed]
  7. Salicylic acid attenuates virulence in endovascular infections by targeting global regulatory pathways in Staphylococcus aureus. Kupferwasser, L.I., Yeaman, M.R., Nast, C.C., Kupferwasser, D., Xiong, Y.Q., Palma, M., Cheung, A.L., Bayer, A.S. J. Clin. Invest. (2003) [Pubmed]
  8. Markedly reduced activity of mutant calcium-sensing receptor with an inserted Alu element from a kindred with familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism. Bai, M., Janicic, N., Trivedi, S., Quinn, S.J., Cole, D.E., Brown, E.M., Hendy, G.N. J. Clin. Invest. (1997) [Pubmed]
  9. Influence of glutathione S-transferase B (ligandin) on the intermembrane transfer of bilirubin. Implications for the intracellular transport of nonsubstrate ligands in hepatocytes. Zucker, S.D., Goessling, W., Ransil, B.J., Gollan, J.L. J. Clin. Invest. (1995) [Pubmed]
  10. Abnormal Na+/H+ antiporter phenotype and turnover of immortalized lymphoblasts from type 1 diabetic patients with nephropathy. Ng, L.L., Davies, J.E., Siczkowski, M., Sweeney, F.P., Quinn, P.A., Krolewski, B., Krolewski, A.S. J. Clin. Invest. (1994) [Pubmed]
  11. Plasma and tissue distribution of adriamycin in patients with pelvic cancer. Piazza, E., Natale, N., Trabattoni, A., Mariscotti, C., Mosca, L., Libretti, A., Ottolenghi, L., Morasca, L. Tumori. (1981) [Pubmed]
  12. Effect of dietary manipulations (fasting, hypocaloric feeding, and subsequent refeeding) on rat muscle energetics as assessed by nuclear magnetic resonance spectroscopy. Pichard, C., Vaughan, C., Struk, R., Armstrong, R.L., Jeejeebhoy, K.N. J. Clin. Invest. (1988) [Pubmed]
  13. Inhibition of nonlysosomal calcium-dependent proteolysis by glycine during anoxic injury of rat hepatocytes. Nichols, J.C., Bronk, S.F., Mellgren, R.L., Gores, G.J. Gastroenterology (1994) [Pubmed]
  14. Effects of nonenzymatic glycosylation of mesangial matrix on proliferation of mesangial cells. Crowley, S.T., Brownlee, M., Edelstein, D., Satriano, J.A., Mori, T., Singhal, P.C., Schlondorff, D.O. Diabetes (1991) [Pubmed]
  15. The anticonvulsant gabapentin (neurontin) does not act through gamma-aminobutyric acid-B receptors. Jensen, A.A., Mosbacher, J., Elg, S., Lingenhoehl, K., Lohmann, T., Johansen, T.N., Abrahamsen, B., Mattsson, J.P., Lehmann, A., Bettler, B., Bräuner-Osborne, H. Mol. Pharmacol. (2002) [Pubmed]
  16. Adeno-associated virus mediates long-term gene transfer and delivery of chondroprotective IL-4 to murine synovium. Watanabe, S., Imagawa, T., Boivin, G.P., Gao, G., Wilson, J.M., Hirsch, R. Mol. Ther. (2000) [Pubmed]
  17. Mechanism of nitric oxide-induced vasodilatation: refilling of intracellular stores by sarcoplasmic reticulum Ca2+ ATPase and inhibition of store-operated Ca2+ influx. Cohen, R.A., Weisbrod, R.M., Gericke, M., Yaghoubi, M., Bierl, C., Bolotina, V.M. Circ. Res. (1999) [Pubmed]
  18. A role for cofilin in the activation of store-operated calcium entry by de novo conformational coupling in human platelets. Redondo, P.C., Harper, M.T., Rosado, J.A., Sage, S.O. Blood (2006) [Pubmed]
  19. Membrane lipid composition and vesicle size modulate bilirubin intermembrane transfer. Evidence for membrane-directed trafficking of bilirubin in the hepatocyte. Zucker, S.D., Goessling, W., Zeidel, M.L., Gollan, J.L. J. Biol. Chem. (1994) [Pubmed]
  20. Increased entry into the IFN-gamma effector pathway by CD4+ T cells selected by I-Ag7 on a nonobese diabetic versus C57BL/6 genetic background. Koarada, S., Wu, Y., Ridgway, W.M. J. Immunol. (2001) [Pubmed]
  21. Sulfonylurea mimics the effect of glucose in inducing large amplitude oscillations of cytoplasmic Ca2+ in pancreatic beta-cells. Grapengiesser, E., Gylfe, E., Hellman, B. Mol. Pharmacol. (1990) [Pubmed]
  22. Intracellular Mg2+ and magnesium depletion in isolated renal thick ascending limb cells. Dai, L.J., Quamme, G.A. J. Clin. Invest. (1991) [Pubmed]
  23. Femtomole analysis of prostaglandin pharmaceuticals. McGuffin, V.L., Zare, R.N. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  24. Activation of nicotinic receptor-induced postsynaptic responses to luteinizing hormone-releasing hormone in bullfrog sympathetic ganglia via a Na+-dependent mechanism. Cao, Y.J., Peng, Y.Y. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  25. Differential endocytosis of T and B lymphocyte surface molecules evaluated with antibody-bearing fluorescent liposomes containing methotrexate. Machy, P., Barbet, J., Leserman, L.D. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  26. Extracellular Ca(2+)-dependent potentiation by cocaine of serotonin- and norepinephrine-induced contractions in rat vascular smooth muscle. Watanabe, C., Yamamoto, H., Kobayashi, S., Kanaide, H. Circ. Res. (1993) [Pubmed]
  27. Time-resolved fluorometry in the diagnosis of Leber hereditary optic neuroretinopathy. Huoponen, K., Juvonen, V., Iitiä, A., Dahlen, P., Siitari, H., Aula, P., Nikoskelainen, E., Savontaus, M.L. Hum. Mutat. (1994) [Pubmed]
  28. Molecular determinants of NMDA receptor function in GABAergic neurones of rat forebrain. Plant, T., Schirra, C., Garaschuk, O., Rossier, J., Konnerth, A. J. Physiol. (Lond.) (1997) [Pubmed]
  29. Binding of discoidin domain receptor 2 to collagen I: an atomic force microscopy investigation. Agarwal, G., Kovac, L., Radziejewski, C., Samuelsson, S.J. Biochemistry (2002) [Pubmed]
  30. Chemokine receptor-ligand interactions measured using time-resolved fluorescence. Inglese, J., Samama, P., Patel, S., Burbaum, J., Stroke, I.L., Appell, K.C. Biochemistry (1998) [Pubmed]
  31. Inhibition of cytokine generation and mediator release by human basophils treated with desloratadine. Schroeder, J.T., Schleimer, R.P., Lichtenstein, L.M., Kreutner, W. Clin. Exp. Allergy (2001) [Pubmed]
  32. Quantitation of chondroitin 4-sulfate and chondroitin 6-sulfate in pathologic joint fluid. Shinmei, M., Miyauchi, S., Machida, A., Miyazaki, K. Arthritis Rheum. (1992) [Pubmed]
  33. Effect of inhibition of matrix metalloproteinases on cartilage loss in vitro and in a guinea pig model of osteoarthritis. Sabatini, M., Lesur, C., Thomas, M., Chomel, A., Anract, P., de Nanteuil, G., Pastoureau, P. Arthritis Rheum. (2005) [Pubmed]
  34. Sulfate content and specific glycosaminoglycan backbone of perlecan are critical for perlecan's enhancement of islet amyloid polypeptide (amylin) fibril formation. Castillo, G.M., Cummings, J.A., Yang, W., Judge, M.E., Sheardown, M.J., Rimvall, K., Hansen, J.B., Snow, A.D. Diabetes (1998) [Pubmed]
  35. Nerve growth factor or IL-3 induces more IL-13 production from basophils of allergic subjects than from basophils of nonallergic subjects. Sin, A.Z., Roche, E.M., Togias, A., Lichtenstein, L.M., Schroeder, J.T. J. Allergy Clin. Immunol. (2001) [Pubmed]
  36. Time-resolved fluoroimmunoassay for unconjugated estrogen in urine: comparison with a fluorometric assay for total estrogen and application in an in vitro fertilization program. Thomas, C.M., van den Berg, R.J., Segers, M.F., Geelen, L.M., Hollanders, J.M., Doesburg, W.H., Houx, P.C. Clin. Chem. (1990) [Pubmed]
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