Disease relevance of rimL

• A series of genes encoding polyproteins containing the tobacco vein mottling virus (TVMV) Nla proteinase along with two other reporter genes (those encoding the Escherichia coli acetate kinase (ACK) and Tn9 chloramphenicol, acetyl transferase (CAT) enzymes) were assembled [1].

High impact information on rimL

• The recombinant acetyl transferase has a pH optimum of 7.5, has K(m) values of 10 microM and 8 microM for 10-deacetylbaccatin III and acetyl CoA, respectively, and is apparently regiospecific toward the 10-hydroxyl group of the taxane ring [2].
• This region was sequenced and three open reading frames were found corresponding to the genes ectA (encoding the diaminobutyric acid acetyl transferase), ectB (encoding the diaminobutyric acid aminotransferase) and ectC (encoding the ectoine synthase) [3].
• Nourseothricin (streptothricin) inactivated by a plasmid pIE636 encoded acetyl transferase of Escherichia coli: location of the acetyl group [4].
• Alcohol acetyl transferase (AAT) condenses isoamyl alcohol and acetyl-CoA to produce isoamyl acetate [5].
• Streptothricin acetyl transferase coded for by plasmids pIE636 and pIE637 in Escherichia coli K-12 was found to be located at the inner side of the cytoplasmic membrane [6].

Chemical compound and disease context of rimL

• Nourseothricin, a mixture of several streptothricins, is inactivated by an acetyl transferase produced by Escherichia coli containing the plasmid pIE636 [7].

References