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BLID  -  BH3-like motif containing, cell death inducer

Homo sapiens

Synonyms: BH3-like motif-containing cell death inducer, BRCC2, Breast cancer cell protein 2
 
 
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Disease relevance of BRCC2

  • In human prostate cancer cells (PC-3), BRCC2-induced DNA fragmentation was blocked efficiently by coexpression of the anti-apoptotic molecule, Bcl-X(L) [1].
  • Exogenous expression of BLID correlated with activation of Bax and an increase in cytosolic cytochrome c. BLID mRNA expression was significantly reduced in grade 3 relative to grade 1 and 2 breast cancer (P = 0.023) [2].
 

High impact information on BRCC2

  • Exogenous expression of BRCC2 caused apoptotic cell death in three different cell lines as evidenced by enhanced chromatin condensation, DNA fragmentation, or an enhanced number of cells in the sub-G(1) phase [1].
  • Ectopic expression of BRCC2 cDNA also was found in both the cytosol and mitochondria [1].
  • BRCC2, a novel BH3-like domain-containing protein, induces apoptosis in a caspase-dependent manner [1].
  • An N-terminal deletion mutant of BRCC2 (10.2 kDa, Delta1-16 aa) lacking a BH3-like domain (5-12 aa, LPIEGQEI) or BRCC2 containing a mutant BH3-like domain (leucine 5-->glutamate) failed to induce apoptosis, whereas a C-terminal deletion mutant (6.8 kDa, Delta62-108 aa) retained the apoptotic activity comparable to the full-length BRCC2 [1].
  • Transient transfection of BRCC2 cDNA into PC-3 cells in the presence of a broad-range caspase inhibitor, Z-VAD-fmk (100 microM, 24 h), abrogated DNA fragmentation [1].
 

Biological context of BRCC2

 

Anatomical context of BRCC2

  • Finally, the treatment of HeLa cells with doxorubicin or hydrogen peroxide (H(2)O(2)) led to an increase in the mitochondrial (heavy membrane) level of endogenous BRCC2 (doxorubicin (100 ng/ml), 5 h, approximately 2-fold; H(2)O(2) (200 microM), 2 h, approximately 2-fold) [1].

References

  1. BRCC2, a novel BH3-like domain-containing protein, induces apoptosis in a caspase-dependent manner. Broustas, C.G., Gokhale, P.C., Rahman, A., Dritschilo, A., Ahmad, I., Kasid, U. J. Biol. Chem. (2004) [Pubmed]
  2. The proapoptotic molecule BLID interacts with Bcl-XL and its downregulation in breast cancer correlates with poor disease-free and overall survival. Broustas, C.G., Ross, J.S., Yang, Q., Sheehan, C.E., Riggins, R., Noone, A.M., Haddad, B.R., Seillier-Moiseiwitsch, F., Kallakury, B.V., Haffty, B.G., Clarke, R., Kasid, U.N. Clin. Cancer Res. (2010) [Pubmed]
 
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