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Gene Review

Nsf2  -  N-ethylmaleimide-sensitive factor 2

Drosophila melanogaster

Synonyms: CG33101, CG9931, DmNSF2, Dmel\CG33101, N-ethylmaleimide-sensitive fusion protein 2, ...
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High impact information on Nsf2

  • We now report the identification of a second homolog of NSF, called dNSF-2 within this species and report evidence that this ubiquitous and widely utilized fusion protein belongs to a multigene family [1].
  • Drosophila that express a neural transgene encoding a dominant-negative form of NSF2 show an 80% reduction in the size of releasable synaptic vesicle pool, but no change in the number of vesicles in nerve terminal boutons [2].
  • Using a combination of genetics, pharmacology, and imaging we find a substantial reduction in vesicle mobility within the nerve terminal boutons of Drosophila NSF2 mutant larvae [2].
  • Here we report the identification of the Drosophila homologue of syntaxin 16. dsyntaxin 16 binds SNAP in a concentration-dependent fashion and genetically interacts with NSF2 [3].
  • A genetic screen for suppressors of Drosophila NSF2 neuromuscular junction overgrowth [4].

Biological context of Nsf2


Anatomical context of Nsf2

  • Intriguingly, we also found that expression of mutant dNSF2 induced pronounced overgrowth of the neuromuscular junction and some misrouting of axons [6].
  • Results of this analysis indicate that dNSF1 function is required in the nervous system beginning at the adult stage of development and that dNSF2 function is required in mesoderm beginning at the first instar larval stage of development [5].
  • Interaction of cytoskeleton genes with NSF2-induced neuromuscular junction overgrowth [7].

Other interactions of Nsf2

  • First, we tested for loss-of-function interaction and, indeed, we found that the combination of flies heterozygous for Act5C and NSF2 alleles led to reduced viability [7].


  1. Identification of a second homolog of N-ethylmaleimide-sensitive fusion protein that is expressed in the nervous system and secretory tissues of Drosophila. Boulianne, G.L., Trimble, W.S. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  2. Synaptic Vesicle Mobility and Presynaptic F-Actin Are Disrupted in a N-ethylmaleimide-sensitive Factor Allele of Drosophila. Nunes, P., Haines, N., Kuppuswamy, V., Fleet, D.J., Stewart, B.A. Mol. Biol. Cell (2006) [Pubmed]
  3. Drosophila syntaxin 16 is a Q-SNARE implicated in Golgi dynamics. Xu, H., Boulianne, G.L., Trimble, W.S. J. Cell. Sci. (2002) [Pubmed]
  4. A genetic screen for suppressors of Drosophila NSF2 neuromuscular junction overgrowth. Laviolette, M.J., Nunes, P., Peyre, J.B., Aigaki, T., Stewart, B.A. Genetics (2005) [Pubmed]
  5. Partitioning of N-ethylmaleimide-sensitive fusion (NSF) protein function in Drosophila melanogaster: dNSF1 is required in the nervous system, and dNSF2 is required in mesoderm. Golby, J.A., Tolar, L.A., Pallanck, L. Genetics (2001) [Pubmed]
  6. Dominant-negative NSF2 disrupts the structure and function of Drosophila neuromuscular synapses. Stewart, B.A., Mohtashami, M., Rivlin, P., Deitcher, D.L., Trimble, W.S., Boulianne, G.L. J. Neurobiol. (2002) [Pubmed]
  7. Interaction of cytoskeleton genes with NSF2-induced neuromuscular junction overgrowth. Peyre, J.B., Seabrooke, S., Randlett, O., Kisiel, M., Aigaki, T., Stewart, B.A. Genesis (2006) [Pubmed]
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