High impact information on Doa

• Mutations at the Darkener of apricot (Doa) locus of Drosophila cause roughened eyes and increase transcript accumulation from the retrotransposon copia up to fourfold [1].
• Doa function is also critical to Drosophila eye development, because the organization and development of pigment cells, bristles, and photoreceptors are affected in various mutant classes [1].
• The Doa locus encodes a member of a new protein kinase family and is essential for eye and embryonic development in Drosophila melanogaster [1].
• Examination of pre-mRNAs regulated similarly to dsx shows that the requirement for Doa is substrate specific [2].
• Mutations in Doa are dominant suppressors or enhancers and are recessive lethals [3].

Biological context of Doa

• Doa mutations disrupt sex-specific splicing of doublesex pre-mRNA, a key regulator of sex determination, by affecting the phosphorylation of one or more proteins in the female-specific splicing enhancer complex [2].
• Mosaic analysis in both the soma and germline demonstrates that Doa function is essential for cell viability [4].
• We identified alleles of oo18 RNA binding protein (orb) and Darkener of apricot (Doa), which had previously been shown to exhibit oogenesis defects [5].
• Rare Doa mutant homozygotes escaping lethality demonstrate extreme phenotypic suppression of wa and enhancement of wsp55 [3].
• Finally, we show that homozygosity for Doa mutations increases the accumulation of copia transcripts from the population of elements in the genome [6].

Anatomical context of Doa

• Identification and analysis of mutations in bob, Doa and eight new genes required for oocyte specification and development in Drosophila melanogaster [5].
• RESULTS: Here we provide evidence for active chromosomal presence of DOA kinase in Drosophila salivary glands, and increased abundance of DOA at eleven specific loci of polythene chromosomes where it can be involved in on-site control of splicing process [7].

Other interactions of Doa

• Mosaic suppressor, a gene in Drosophila that modifies retrotransposon expression and interacts with zeste [8].

Analytical, diagnostic and therapeutic context of Doa

• METHODS: DOA kinase was detected on squash preparations of polythene chromosomes using anti-DOA antibodies in combination with indirect immunofluorescence [7].

References